tailieunhanh - Báo cáo khóa học: Antimicrobial activities of heparin-binding peptides

Antimicrobial peptides are effector molecules of the innate immune system. We recently showed that the human anti-microbial peptidesa-defensin and LL-37 bind to glycos-aminoglycans (heparin and dermatan sulphate). Here we demonstrate the obverse, . structural motifs associated with heparin affinity (cationicity, amphipaticity, and con-sensus regions)may confer antimicrobial properties to a given peptide. Thus, heparin-binding peptides derived from laminin isoforms, von Willebrand factor, vitronectin, pro-tein C inhibitor, and fibronectin, exerted antimicrobial activities against Gram-positive and Gram-negative bac-teria. . | Eur. J. Biochem. 271 1219-1226 2004 FEBS 2004 doi Antimicrobial activities of heparin-binding peptides Emma Andersson1 Victoria Rydengard1 Andreas Sonesson1 Matthias Morgelin2 Lars Bjorck2 and Artur Schmidtchen1 1 Department of Medical Microbiology Dermatology and Infection Section for Dermatology 2Department of Cell and Molecular Biology Section for Molecular Pathogenesis Lund University Biomedical Center Sweden Antimicrobial peptides are effector molecules of the innate immune system. We recently showed that the human antimicrobial peptides a-defensin and LL-37 bind to glycosaminoglycans heparin and dermatan sulphate . Here we demonstrate the obverse . structural motifs associated with heparin affinity cationicity amphipaticity and consensus regions may confer antimicrobial properties to a given peptide. Thus heparin-binding peptides derived from laminin isoforms von Willebrand factor vitronectin protein C inhibitor and fibronectin exerted antimicrobial activities against Gram-positive and Gram-negative bacteria. Similar results were obtained using heparin-binding peptides derived from complement factor C3 as well as consensus sequences for heparin-binding Cardin and Weintraub motifs . These sequence motifs and additional peptides also killed the fungus Candida albicans. These data will have implications for the search for novel antimicrobial peptides and utilization of heparin-protein interactions should be helpful in the identification and purification of novel antimicrobial peptides from complex biological mixtures. Finally consensus regions may serve as templates for de novo synthesis of novel antimicrobial molecules. Keywords antimicrobial cathelicidin defensin heparin binding glycosaminoglycan. Multicellular organisms express a blend of antimicrobial peptides AMP which are ubiquitously distributed at biological boundaries prone to infection. These peptides originally described in silk worms 1 occur in animals ranging from