tailieunhanh - Báo cáo khóa học: Isolation and characterization of the Xenopus HIVEP gene family

The HIVEP gene family encodes for very large sequence-specific DNAbinding proteins containing multiple zinc fingers. Three mammalian paralogous genes have been identified, HIVEP1,-2and -3, as well as the closely related Drosophilagene,Schnurri. These genes have been found to directly participate in the transcriptional regulation of a variety of genes. Mammalian HIVEP members have been implicated in signaling by TNF-aand in the positive selec-tion of thymocytes, whileSchnurrihas been shown to be an essential component of theTGF-bsignalingpathway. . | Eur. J. Biochem. 271 1135-1144 2004 FEBS 2004 doi Isolation and characterization of the Xenopus HIVEP gene family Ulrike Diirr1 Kristine A. Henningfeld1 Thomas Hollemann1 Walter Knochel2 and Tomas Pieler1 1Abteilung Entwicklungsbiochemie Universitat Gottingen Germany 2Abteilung Biochemie Universitat Ulm Germany The HIVEP gene family encodes for very large sequencespecific DNA bidding proleiss contilining multiple ínc fingers. Three mammalian paralogous genes have been identified HIVEP1 -2 and -3 as well as the closely related Drosophila gene Schnurri. These genes have been found to directly participate in the transcriptional regulation of a variety of genes. Mammalian HIVEP members have been implicated in signaling by TNF-a and in the positive selection of thymocytes while Schnurri has been shown to be an essential component of the TGF-b signaling pathway. In this study we describe the isolation of Xenopus HIVEP1 as well as partial cDNAs of HIVEP2 and -3. Analysis of the temporal and spatial expression of the XHIVEP transcripts during early embryogenesis revealed ubiquitous expression of the transcripts. Assays using Xenopus oocytes mapped XHIVEP1 domains that are responsible for nuclear export and import activity. The DNAbinding specificity of XHIVEP was characterized using a PCR-mediated selection and gel mobility shift assays. Keywords DNA bíndíng Schnurri Xenopus zinc finger. The HIVEP family of zinc finger proteins regulates a diverse array of developmental and biological processes through direct DNAbinding as well as interaction with other transcription factors and components of signal transduction pathways 1 2 . Representative members include three human genes HIVEP1 also called ZAS1IShn1ỊMBP1l PRDII-BF1 3-6 HIVEP2 ZAS2IShn2l Mbp2 7 8 and HIVEP3 ZAS3 Shn3 7 9 as well as the corresponding mouse homologues aACRYBP1 10 11 MIBP1 12 and KRC 13 . Schnurri Shn a distantly related ortholog from Drosophila which is most closely .