tailieunhanh - Báo cáo khóa học: Characterization of novel structural features in the lipopolysaccharide of nondisease associated nontypeable Haemophilus influenzae

NontypeableHaemophilus influenzae(NTHi) is a common commensal of the human upper respiratory tract and is associatedwithotitismedia in children. The structures of the oligosaccharide portions of NTHi lipopolysaccharide (LPS) from several otitis media isolates are now well characterized but it is not knownwhether there are structural differences in LPSfrom colonizing, nondisease associated strains. | Eur. J. Biochem. 271 941-953 2004 FEBS 2004 doi l Characterization of novel structural features in the lipopolysaccharide of nondisease associated nontypeable Haemophilus influenzae Malin K. Landerholm1 Jianjun Li2 James C. Richards2 Derek W. Hood3 E. Richard Moxon3 and Elke K. H. Schweda1 1 Clinical Research Centre Karolinska Institutet and University College of South Stockholm NOVUM Huddinge Sweden .Institute for Biological Sciences National Research Council of Canada Ottawa Ontario Canada 3Molecular Infectious Diseases Group University of Oxford Department of Paediatrics Weatherall Institute of Molecular Medicine John Radcliffe Hospital Oxford UK Nontypeable Haemophilus influenzae NTHi is a common commensal of the human upper respiratory tract and is associated with otitis media in children. The structures of the oligosaccharide portions of NTHi lipopolysaccharide LPS from several otitis media isolates are now well characterized but it is not known whether there are structural differences in LPS from colonizing nondisease associated stems. Sfruc-tural analysis of LPSfrom nondisease associated NTHi strains -1 and -6 has been achieved by the application of high-field NMR techniques ESI-MS ESI-MSn capillary electrophoresis coupled to ESI-MS composition and linkage analyses on O-deacylated LPS and coee oiigosacchaide material. This is the first study to report structural details on LPS room sri ams taken room the nasopharynx room lienal dry individuals. Both strains express identical structures and contain the common element of H. influenzae LPS L-a-D-Hepp- 1 fi2 - PEtnfi6 -L-a-D-Hepp- 1 fi3 - b-D-Glcp- 1fi4 -L-a-D-Hepp- 1fi5 - PPEtnfi4 -a-Kdop- 2fi6 -lipid A in which each heptose is elongated by a single hexose residue with no further oligosaccharide extensions. In the ma or Hex3 glycoform. the terminal Hepp residue HepIII is substituted at the O-2 position by a b-D-Galp residue and the central Hepp residue HepII is substituted at .