tailieunhanh - Báo cáo khoa học: Strategies to cover microbial proteomes

The aim of our studies is to get access to as much as possible of the protein species present in the proteome under analysis. Already at the low complexity level of proteasomes it becomes clear that proteins are massively modified. There are about 70 spots representing different protein forms from which only some of them were identified at the protein species level. | Abstracts 4. New Analytical and Diagnostic Approaches Bioinformatics and Proteomics Strategies to cover microbial proteomes P. R. Jungblut Core Facility Protein Analysis Max Planck Institute for Infection Biology Berlin Germany. E-mail jungblut@ The aim of our studies is to get access to as much as possible of the protein species present in the proteome under analysis. Already at the low complexity level of proteasomes it becomes clear that proteins are massively modified. There are about 70 spots representing different protein forms from which only some of them were identified at the protein species level. The mycobacterial proteome of a cell extract of late growth phase state revealed by 2-DE MS 1800 spots with 379 different proteins identified 10 of the predicted genes . Isotope coded affinity tag ICAT -LC MS resulted in 619 15 of predicted genes identified proteins. Because of the overlap of 158 proteins showing the high complementarity of 2-DE MS and ICAT-LC MS the prote-ome is covered at present by 21 . For Helicobacter pylori we revealed with a pre-fractionation strategy about 520 different proteins 33 of predicted proteome . One of the proteins determined as vaccine candidate was successful as vaccine in animal models and is now in a clinical study. A proteome 2-DE database was established http 2D-PAGE which contains data of more than 10 different bacterial species. Proteomics does not end with the identification of a protein. At present identification at the protein species level which needs 100 sequence coverage is very time-consuming and affords further technology development. Today the enormous information content of a 2-DE gel is only accessible to a small extent. Targeted peptide-centric protemics a versatile tool for quantitative proteomics J. Vandekerckhove L. Martens J. Pinxteren P. Van Damme X. Hanoulle B. Ghcsquicrc A. Staes J. Van Damme E. Timmerman M. Goethals H. Demol K. Hugelier and

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