tailieunhanh - Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B

The staphylococcal enterotoxins produced byStaphylococcus aureusare associated with pyrogenic response in humans and primates. This study investigates the role of NADPH oxidase and nuclear factor-kappa B (NF-jB) on enterotoxin staphylococcal enterotoxin C1 (SEC1)-induced pyrogenic cytokine production in human peripheral blood mononuclear cells (PBMC). | ỊFEBS Journal Staphylococcal enterotoxin Ci-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B Chun-Li Su1 Chun-Chun Cheng2 Mao-Tsun Lin3 Hsiao-Chun Yeh2 Meng-Chou Lee2 Jenq-Chang Lee4 and Shen-Jeu Won2 1 Department of Nursing Chang Jung Christian University Tainan Taiwan 2 Department of Microbiology and Immunology MedicalCollege NationalCheng Kung University Tainan Taiwan 3 Department of MedicalResearch Chi-Mei MedicalCenter Tainan Taiwan 4 Department of Surgery MedicalCollege NationalCheng Kung University Tainan Taiwan Keywords human peripheral blood mononuclear cells NADPH oxidase NF-kB pyrogenic cytokine staphylococcal enterotoxin C1 Correspondence . Won Department of Microbiology and Immunology MedicalCollege National Cheng Kung University no. 1 Ta-Hsueh Road Tainan 701 Taiwan Fax 886 6 2082705 Tel 886 6 2744435 E-mail a725@ Received 2 April2007 revised 17 May 2007 accepted 22 May 2007 doi The staphylococcal enterotoxins produced by Staphylococcus aureus are associated with pyrogenic response in humans and primates. This study investigates the role of NADPH oxidase and nuclear factor-kappa B NF-kB on enterotoxin staphylococcal enterotoxin C1 SEC1 -induced pyrogenic cytokine production in human peripheral blood mononuclear cells PBMC . The results indicate that the febrile response to the supernatant fluids of SEC1-stimulated PBMC in rabbits was in parallel with the levels of interleukin-1 b and interleukin-6 in the supernatants. The release of interleukin-1 b and interleukin-6 nuclear translocation of NF-kB and its DNA binding activity in the SEC1-stimulated PBMC were time-dependent and were completely eliminated by pyrrolidine dithiocarbamate or SN-50 NF-kB inhibitors . The release of reactive oxygen species in the supernatants and translocation of the NADPH oxidase p47phox subunit to the plasma membrane of .