tailieunhanh - Báo cáo khoa học: Cartducin stimulates mesenchymal chondroprogenitor cell proliferation through both extracellular signal-regulated kinase and phosphatidylinositol 3-kinase⁄Akt pathways

Cartducin, a paralog of Acrp30⁄adiponectin, is a secretory protein pro-duced by both chondrogenic precursors and proliferating chondrocytes, and belongs to a novel C1q family of proteins. We have recently shown that cartducin promotes the growth of both mesenchymal chondroprogeni-tor cells and chondrosarcoma-derived chondrocytic cells in vitro. | ềFEBS Journal Cartducin stimulates mesenchymal chondroprogenitor cell proliferation through both extracellular signal-regulated kinase and phosphatidylinositol 3-kinase Akt pathways Hironori Akiyama1 2 Souhei Furukawa2 Satoshi Wakisaka1 and Takashi Maeda1 1 Department of Anatomy and Cell Biology Graduate Schoolof Dentistry Osaka University Japan 2 Department of Radiology Graduate Schoolof Dentistry Osaka University Japan Keywords cartducin chondroprogenitor cells MAPK PI3K Akt proliferation Correspondence T. Maeda Department of Anatomy and Cell Biology Graduate Schoolof Dentistry Osaka University 1-8 Yamadaoka Suita Osaka 565-0871 Japan Fax 81 6 6879 2875 Tel 81 6 6879 2874 E-mail tmaeda@ Received 20 November 2005 revised 20 February 2006 accepted 20 March 2006 doi Cartducin a paralog of Acrp30 adiponectin is a secretory protein produced by both chondrogenic precursors and proliferating chondrocytes and belongs to a novel C1q family of proteins. We have recently shown that cartducin promotes the growth of both mesenchymal chondroprogenitor cells and chondrosarcoma-derived chondrocytic cells in vitro. However the cartducin-signaling pathways responsible for the regulation of cell proliferation have not been documented. In this study we examined whether cartducin exists in serum and further investigated the intracellular signaling pathways stimulated by cartducin in mesenchymal chondroprogenitor cells. Western blot analysis showed that unlike Acrp30 adiponectin cartducin was undetectable in mouse serum. Next mesenchymal chondroprogenitor N1511 cells were stimulated with cartducin and three major groups of mitogen-activated protein kinase MAPK pathways and the phosphatidylinositol 3-kinase PI3K Akt signaling pathway were examined. Cartducin activated extracellular signal-regulated kinase 1 2 ERK1 2 and Akt but not c-jun N-terminal kinase JNK nor p38 MAPK. The MEK1 2 inhibitor U0126 blocked cartducin-stimulated ERK1 2