tailieunhanh - Báo cáo khoa học: Reconstitution in vitro of the GDP-fucose biosynthetic pathways of Caenorhabditis elegans and Drosophila melanogaster

The deoxyhexose sugar fucose has an important fine-tuning role in regula-ting the functions of glycoconjugates in disease and development in mam-mals. The two genetic model organismsCaenorhabditis elegans and Drosophila melanogasteralso express a range of fucosylated glycans, and the nematode particularly has a number of novel forms. | iFEBS Journal Reconstitution in vitro of the GDP-fucose biosynthetic pathways of Caenorhabditis elegans and Drosophila melanogaster Simone Rhomberg1 Christina Fuchsluger1 Dubravko Rendic1 Katharina Paschinger1 Verena Jantsch2 Paul Kosma1 and Iain B. H. Wilson1 1 Department fur Chemie Universitat fur Bodenkultur Vienna Austria 2 Abteilung fur Chromosomenbiologie Vienna Biocenter II Austria Keywords Caenorhabditis Drosophila GDP-fucose biosynthesis GDP-keto-6-deoxymannose 3 5-epimerase 4-reductase GDP-mannose dehydratase Correspondence I. B. H. Wilson Department fur Chemie Universitat fur Bodenkultur Muthgasse 18 A-1190 Vienna Austria Fax 43 1 36006 6059 Tel 43 1 36006 6541 E-mail Database The nucleotide sequences of C. elegans and D. melanogaster gmd and ger cDNA have been submitted to the EMBL database under accession numbers AM231683 AM231684 AM231685 AM231686 AM231687 and AM231688 Received 30 November 2005 revised 17 February 2006 accepted 20 March 2006 doi The deoxyhexose sugar fucose has an important fine-tuning role in regulating the functions of glycoconjugates in disease and development in mammals. The two genetic model organisms Caenorhabditis elegans and Drosophila melanogaster also express a range of fucosylated glycans and the nematode particularly has a number of novel forms. For the synthesis of such glycans the formation of GDP-fucose which is generated from GDP-mannose in three steps catalysed by two enzymes is required. By homology we have identified and cloned cDNAs encoding these two proteins GDP-mannose dehydratase GMD EC and GDP-keto-6-deoxymannose 3 5-epimerase 4-reductase GER or FX protein EC from both Caenorhabditis and Drosophila. Whereas the nematode has two genes encoding forms of GMD gmd-1 and gmd-2 and one GER-encoding gene ger-1 the insect has like mammalian species only one homologue of each gmd and gmer . This compares to the presence of two forms of both .