tailieunhanh - Báo cáo khóa học: A new UV-B absorbing mycosporine with photo protective activity from the lichenized ascomycete Collema cristatum

Anovel photoprotectivemycosporinewas isolated fromthe lichenized ascomyceteCollema cristatum. Biological activity was measured in terms of protection against UV-B induced membrane destruction and pyrimidine dimer formation in cultured human keratinocytes, and prevention of UV-B induced erythema. It was found that the pure isolated compound prevented UV-B induced cell destruction in a dose-dependent manner, that the compound partially pre-vented pyrimidine dimer formation and completely preven-ted UV-B induced erythema when applied to the skin prior to irradiation | Eur. J. Biochem. 271 780-784 2004 FEBS 2004 doi A new UV-B absorbing mycosporine with photo protective activity from the lichenized ascomycete Collema cristatum Avital Torres1 Malka Hochberg4 Inna Pergament1 Reem Smoum1 Valerie Niddam2 Valery M. Dembitsky1 Marina Temina5 Inka Dor3 Ovadia Lev3 Morris Srebnik1 and Claes D. Enk4 1 Department of Medicinal Chemistry and Natural Products School of Pharmacy Hebrew University of Jerusalem Israel 2Teva Pharmaceutical Industries Ltd Petach Tikva Israel 3Division of Environment Science The Fredy and Nadine Herrmann Graduate School of Applied Sciences The Hebrew University of Jerusalem Israel 4Department of Dermatology Hadassah University Hospital Jerusalem Israel 5Institute of Ecology Haifa University Israel A novel photo protective mycosporine was isolated from the lichenized ascomycete Collema cristatum. Biological activity was measured in terms of protection against UV-B induced membrane destruction and pyrimidine dimer formation in cultured human keratinocytes and prevention of UV-B induced erythema. It was found that the pure isolated compound prevented UV-B induced cell destruction in a dose-dependent manner that the compound partially prevented pyrimidine dimer formation and completely prevented UV-B induced erythema when applied to the skin prior to irradiation. Keywords lichenized ascomycete Collema cristatum myco-sporine photo protective activity UV-B. Lichens are symbiotic organisms of fungi algae and or cyanobacteria that synthesize numerous secondary metabolites which comprise aliphatic aromatic terpenoic and halogenated compounds. Several species absorb strongly in the UV-B range 1 2 4 . Lichens and their metabolites have been demonstrated to possess numerous biological activities including antiviral antibacterial antitumor antiallergic antiherbivore and enzyme inhibitory activity. Some active lichen substances are used in the pharmaceutical industry V. M. Dembitsky unpublished .