tailieunhanh - Báo cáo khóa học: Expression of mitochondrial HMGCoA synthase and glutaminase in the colonic mucosa is modulated by bacterial species

The expression of the colonic mitochondrial 3-hydroxy 3-methyl glutaryl CoA (mHMGCoA) synthase,a key con-trol site of ketogenesis from butyrate,is lower in germ-free (GF) than in conventional (CV) rats. In contrast,the activity of glutaminase is higher. The objective of this study was to investigate whether the intestinal flora can affect gene expression through short chain fatty acid (SCFA) and butyrate production. GF rats were inoculated with a con-ventional flora (Ino-CV) orwitha bacterial strainproducing butyrate (Clostridium paraputrificum,Ino-Cp) or not (Bifidobacterium breve,Ino-Bb). . | Eur. J. Biochem. 271 A Ạ 2004 FEBS 2003 doi Expression of mitochondrial HMGCoA synthase and glutaminase in the colonic mucosa is modulated by bacterial species Claire Cherbuy1 Claude Andrieux2 Edith Honvo-Houeto1 Muriel Thomas1 Catherine Ide1 Nathalie Druesne1 Catherine Chaumontet1 Beatrice Darcy-Vrillon1 and Pierre-Henri Duee1 1 Laboratoire de Nutrition et Sécurité Alimentaire and 2 Unite d Ecologie et de Physiologie du Systeme Digestif Institut National de la Recherche Agronomique Jouy-en-Josas France The expression of the colonic mitochondrial 3-hydroxy 3-methyl glutaryl CoA mHMGCoA synthase a l y control site of ketogenesis from butyrate is lower in germ-l ree GF than in conventional CV rats. In contrast Ihe acii vily of glutaminase is higher. The objective of this study was to investigate whether the intestinal flora can affect gene expression through short chain fatty acid SCFA and butyrate production. GF rats were inoculated with a conventional flora Ino-CV or with a bacterial strain producing butyrate Clostridium paraputrificum ono-Cp or not Bifidobacterium breve Ĩno-Bb . In the Ino-CV rats mHMGCoA synthase expression was restored to the CV values 2 days after the inoculation its. concornt Ian tty with SCFA production. In the Ino-Cp group but not m the ino-Bb group mHMGCdA eonsfare Odd ghamsOnere were expressed at the level observed in the CV rats. These data suggest that the intestinal flora thl OLlsh 01 1X1 production mild co It too I the 6X111 0011 oi ooloníc mHMGCoA synthase and glutaminase. These modifications in gene expression by butyrate in vivo seem unrelated to a modification of histone acetylation. Keywords butyrate colonocyte glutaminase mHMGCoA synthase intestinal flora. The distal section of the gastrointestinal tract contains a large and complex population of bacterial species. Experimental evidence derived from animal models indicates a close relationship between the colonic microflora and the intestinal .