tailieunhanh - Báo cáo khóa học: Active-site residues and amino acid specificity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB

In bacteria, coenzyme A is synthesized in five steps from D-pantothenate. The Dfp flavoprotein catalyzes the synthe-sis of the coenzyme A precursor 4¢-phosphopantetheine from 4¢-phosphopantothenate and cysteine using the cofac-tors CTP and flavine mononucleotide via the phospho-peptide-like compound 4¢-phosphopantothenoylcysteine. The synthesis of 4¢-phosphopantothenoylcysteine is cata-lyzedby theC-terminal CoaBdomainofDfp andoccurs via the acyl-cytidylate intermediate 4¢-phosphopantothenoyl-CMP in two half reactions. . | Eur. J. Biochem. 271 163-172 2004 FEBS 2003 doi Active-site residues and amino acid specificity of the bacterial 4 -phosphopantothenoylcysteine synthetase CoaB Thomas Kupke Lehrstuhl fur Mikrobielle Genetik Universitat Tubingen Tubingen Germany In bacteria coenzyme A is synthesized in five steps from D-pantothenate. The Dfp flavoprotein catalyzes the synthesis of the coenzyme A precursor 4 -phosphopantetheine from 4 -phosphopantothenate and cysteine using the cofactors CTP and flavine mononucleotide via the phosphopeptide-like compound 4 -phosphopantothenoylcysteine. The synthesis of 4 -phosphopantothenoylcysteine is catalyzed by the C-terminal CoaB domain of Dfp and occurs via the acyl-cytidylate intermediate 4 -phosphopantothenoyl-CMP in two half reactions. In this new study the molecular characterization of the CoaB domain is continued. In addition to the recently described residue Asn210 two more active-site residues Arg206 and Ala276 were identified and shown to be involved in the second half reaction of the R -4 -phospho-N-pantothenoylcysteine synthetase. The proposed intermediate of the R -4 -phospho-N-panto-thenoylcysteine synthetase reaction 4 -phosphopantothe- noyl-CMP was characterized by MALDI-TOF MS and it was shown that the intermediate is copurified with the mutant His-CoaB N210H K proteins. Therefore His-CoaB N210H and His-CoaB N210K will be of interest to elucidate the crystal structure of CoaB complexed with the reaction intermediate. Wild-type His-CoaB is not absolutely specific for cysteine and can couple derivatives of cysteine to 4 -phosphopantothenate. However no phosphopeptide-like structure is formed with serine. Molecular characterization of the temperature-sensitive Escherichia coli dfp-1 mutant revealed that the residue adjacent to Ala276 Ala275 of the strictly conserved AAVAD 275-279 motif is exchanged for Thr. Keywords coenzyme A biosynthesis 4 -phosphopantethe-ine 4 -phosphopantothenoylcysteine .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• breast cancer
• medicine
• chemical reaction
• Elements of Chemical Reaction Engineering
• Chemical Reaction Engineering
• Hóa học và ứng dụng
• Kỹ thuật hóa học
• Phản ứng hóa học
• Kỹ thuật phản ứng hóa học
• The chemical vapor deposition condition
• The electrochemically catalytic efficiency
• Hydrogen evolution reaction in MoS2 nanoparticles
• Hydrogen evolution reaction
• Metal organic chemical vapor deposition
• Reaction Kinetics
• Heterogeneous Catalysis
• Elementary Reactions
• Microkinetic Analysis
• Nonisothermal Reactors
• Catalytic Reactions
• Quantum chemical modeling mechanism
• Semiempirical method RM1
• Quantum chemical study
• Half chair shape
• Diels Alder reaction
• Chemical reaction engirneering
• Hoá phân tích
• Giải pháp phản ứng hóa học
• Radiation on MHD casson fluid flow
• Chemical reaction effects on MHD casson fluid flow
• Porous medium with suction injection
• The occurrence of suction/injection
• The central PDEs
• Radiation of MHD visco elastic fluid flow over
• Chemical reaction on heat of MHD visco elastic
• Mass transfer of MHD visco elastic fluid flow
• Exponentially stretching sheet through porous medium
• Radiation on the boundary layer MHD casson fluid
• Chemical reaction effects on the boundary layer MHD casson fluid
• Vertical plate embedded in the porous medium
• Casson fluid flow free convection boundary layer fl
• Extreme learning machine
• Artificial chemical reaction optimization algorithm
• Single hidden layer feed forward neural network
• Learning algorithm
• Chemical reaction kinetic
• Adsorption kinetics
• Pseudo first order model
• Pseudo second order model
• General kinetic model
• Dried Probiotics
• Industrial Processes
• Membrane Separation
• Wastewater
• Maillard Reaction
• Pros and Cons
• Chemical kinetics
• Reactant concentration
• Reaction rates and stoichiometry
• Action of catalysts
• Surface area
• Chemical kinetics
• Introduction to general
• Chemical bonds
• Chemical reactions
• Reaction rates
• Chemical equilibrium
• Nuclear chemistry
• Nuclear engineering and technology
• Correlation between rare earth elements
• The chemical interactions of HT9 cladding
• FueleCladding Chemical Interaction
• Higher reaction thickness
• Summary of Chemical PhD Thesis
• Theoretical and Physical Chemistry
• CO hydrogenation reaction
• Ni Cu bimetallic catalyst system
• Density functional method
• Chemical method
• Lecture General Chemistry 1
• Bài giảng Hóa học đại cương 1
• Balance chemical equations
• Calculate the percent yield of a reaction
• Chemical formulas
• Chemical equations
• organic chemistry
• hóa hữu cơ
• công nghệ hóa học
• Chemical Engineering
• common reaction mechanisms
• Chromatography in the petroleum industry
• oxidation
• tumor cells
• BMC Bioinformatics
• Flux balance analysis
• Linear programming
• Design of metabolic network
• Constraint based model
• Growth rate
• Production rate
• Smaller reaction network
• Luận án Tiến sĩ
• Physical chemistry
• Fabbricated electrocatalysts
• Oxygen evolution reaction
• Proton exchange membrane
• cytotoxicity
• Atomic nucleus
• electrons
• Chemical compounds
• Electrochemistry