tailieunhanh - Báo cáo khóa học: The lysozyme of the starfishAsterias rubens A paradigmatic typei lysozyme

On the basis of a partial N-terminal sequence, Jolle`s and Jolle`s [Jolle`s, J., & Jolle`s, P. (1975)Eur. J. , 19–23] previously proposed that the lysozyme from the starfishAsterias rubensrepresents a new form of lysozyme, called typei(invertebrate) lysozyme. Indeed, it differed from both the typesc(chicken) and g(goose) known in other animals, as well as from plant and phage lysozymes. Recently, several proteins belonging to the same family have been isolated from protostomes. Here we report the com-plete mature protein sequence and cDNA sequence of the lysozyme from Asterias. . | Eur. J. Biochem. 271 237-242 2004 FEBS 2003 doi The lysozyme of the starfish Asterias rubens A paradigmatic type i lysozyme Sana Bachali1 Xavier Bailly2 Jacqueline Jolles3 Pierre Jolles3 and Jean S. Deutsch1 1 Equipe Developpement et Evolution UMR 7622 Biologie du développement CNRS et Université P M Curie Paris Fracice 2Station Biologique Rorcoff France 3Lrborrtoire der Proteiaer Univerrite Prrir V France On the basis of a partial N-terminal sequence Jollies and Jollies Jollies J. Jollies P. 1975 Eur. J. Biochem. 54 19-23 previously proposed that the lysozyme from the starfish Arteriar rubenr represents a new form of lysozyme called type i invertebrate lysozyme. Indeed it differed from both the types c chicken and g goose known in other animals as well as from plant and phage lysozymes. Recently several proteins belonging to the same family have been isolated from protostomes. Here we report the com plete mature protein sequence and cDNA sequence of the lysozyme from Arteriar. These sequences vindicate the previously proposed homology between the starfish a deuterostome and protostome lysozymes. In addition we present a structural analysis that allows us to postulate upon the function of several conserved residues. Keywordr cDNA invertebrates lysozyme starfish structure. During recent years interest in a new type of lysozyme the invertebrate-type i-type has been growing. In 1996 Jollies et al. 1 published the N-terminal sequences of lysozymes from two coastal bivalves belonging to the genus Mytilur and of four deep-sea bivalves belonging to the genera Bathymodiolur and Calyptogena. This lysozyme represented a model for the digestion of bacteria by the deep-sea bivalves 2 . A similar lysozyme was then described in other bivalves Taper japonica 3 and Chlamyr irlandica 4 5 . These authors noticed the striking similarity between the bivalve lysozyme and another protein the so-called desta-bilase identified in the medicinal leech Hirudo