tailieunhanh - Báo cáo khoa học: Epl1, the major secreted protein of Hypocrea atroviridis on glucose, is a member of a strongly conserved protein family comprising plant defense response elicitors

We used a proteomic approach to identify constitutively formed extracellu-lar proteins of Hypocrea atroviridis(Trichoderma atroviride), a known bio-control agent. The fungus was cultivated on glucose and the secretome was examined by two-dimensional gel electrophoresis. | ễFEBS Journal Epl1 the major secreted protein of Hypocrea atroviridis on glucose is a member of a strongly conserved protein family comprising plant defense response elicitors Verena Seidl1 Martina Marchetti2 Reingard Schandl1 2 Gunter Allmaier2 and Christian P. Kubicek1 1 Research Area Gene Technology and Applied Biochemistry Institute of ChemicalEngineering Vienna University of Technology Austria 2 Institute of ChemicalTechnologies and Analytics Vienna University of Technology Austria Keywords cerato-platanin elicitor Hypocrea Trichoderma plant defense responses Correspondence V. Seidl Research Area Gene Technology and Applied Biochemistry Institute of ChemicalEngineering Vienna University of Technology Getreidemarkt 9 166-5 A-1060 Vienna Austria Fax 43 1 58801 17299 Tel 43 1 58801 17227 E-mail vseidl@ Website http Received 30 March 2006 revised 25 July 2006 accepted 27 July 2006 doi We used a proteomic approach to identify constitutively formed extracellular proteins of Hypocrea atroviridis Trichoderma atroviride a known biocontrol agent. The fungus was cultivated on glucose and the secretome was examined by two-dimensional gel electrophoresis. The two predominant spots were identified by MALDI MS utilizing peptide mass fingerprints and amino acid sequence tags obtained by postsource decay and or high-energy collision-induced dissociation MS MS experiments and turned out to be the same protein 12 629 Da as determined with MS pl probably representing the monomer and the dimer. The corresponding gene was subsequently cloned from H. atroviridis and named epll eliciting plant response-like because it encodes a protein that exhibits high similarity to the cerato-platanin family which comprises proteins such as cerato-plata-nin from Ceratocystis fimbriata f. sp. platani and Snodprotl of Phaeos-phaeria nodorum which have been reported to be involved in plant pathogenesis and elicitation