tailieunhanh - Báo cáo khoa học: A novel Takeout-like protein expressed in the taste and olfactory organs of the blowfly, Phormia regina

In insects, the functional molecules responsible for the taste system are still obscure. The gene for a kDa protein purified from taste sensilla of the blowflyPhormia reginabelongs to a gene family that includestakeout of Drosophila melanogaster. Molecular phylogenetic analysis revealed that the PhormiaTakeout-like protein is most similar to the protein encoded by a member of theDrosophila takeoutgene family, CG14661, whose expression and function have not been identified yet. | ỊFEBS Journal A novel Takeout-like protein expressed in the taste and olfactory organs of the blowfly Phormia regina Kazuyo Fujikawa Keiji Seno and Mamiko Ozaki Department of Applied Biology Faculty of Textile Science Kyoto Institute of Technology Matsugasaki Sakyo-ku Kyoto Japan Keywords chemoreceptor fly olfaction takeout taste Correspondence M. Ozaki Kobe University Rokkodai Nada-ku Kobe 657-8501 Japan Fax 81 803 5718 Tel 81 78 803 5718 E-mail mamiko@ Received 19 December 2005 revised 27 June 2006 accepted 24 July 2006 doi In insects the functional molecules responsible for the taste system are still obscure. The gene for a kDa protein purified from taste sensilla of the blowfly Phormia regina belongs to a gene family that includes takeout of Drosophila melanogaster. Molecular phylogenetic analysis revealed that the Phormia Takeout-like protein is most similar to the protein encoded by a member of the Drosophila takeout gene family CG14661 whose expression and function have not been identified yet. Western blot analyses revealed that Phormia Takeout-like protein was exclusively expressed in antennae and labellum of the adult blowfly in both sexes. Immunohistochemical experiments demonstrated that Takeout-like protein was localized around the lamella structure of the auxiliary cells and in the sensillar lymph of the labellar taste sensillum. In antennae Takeout-like protein was distributed at the base of the olfactory sensilla as well. No significant differences in Takeout-like protein expression were found between the sexes. Our results suggest that Phormia Takeout-like protein is involved in some early events concerned with chemoreception in both the taste and olfactory systems. The contact chemosensilla on the labellum which are major taste organs of the fly are in the form of a hair housing four taste receptor cells and one mechanoreceptor cell 1 . Taste transduction is triggered by the reception of .