tailieunhanh - Báo cáo khoa học: A genetic screen identifies mutations in the yeastWAR1 gene, linking transcription factor phosphorylation to weak-acid stress adaptation

Exposure of the yeastSaccharomyces cerevisiaeto weak organic acids such as the food preservatives sorbate, benzoate and propionate leads to the pronounced induction of the plasma membrane ATP-binding cassette (ABC) transporter, Pdr12p. This protein mediates efflux of weak acid ani-ons, which is essential for stress adaptation. | ễFEBS Journal A genetic screen identifies mutations in the yeast WAR1 gene linking transcription factor phosphorylation to weak-acid stress adaptation Christa Gregori Bettina Bauer Chantal Schwartzf Angelika KrenỊ Christoph Schuller and Karl Kuchler MedicalUniversity Vienna Max F. Perutz Laboratories Department of MedicalBiochemistry Campus Vienna Biocenter Austria Keywords ABC transporter stress response weak organic acids yeast zinc finger Correspondence K. Kuchler MedicalUniversity Vienna Max F. Perutz Laboratories Department of Medical Biochemistry Campus Vienna Biocenter Dr Bohr-Gasse 9 2 A-1030 Vienna Austria Fax 43 1 4277 9618 Tel 43 1 4277 61807 E-mail Present address fUniversite de Nice-Sophia Antipolis Inserm U636 Centre de Biochimie UFR Sciences Parc Valrose Nice France Institute of Biochemistry and Genetics Department of Clinical and Biological Research DKBW Basel Switzerland University of Vienna Max F. Perutz Laboratories Department of Biochemistry Molecular and Cellular Biology Campus Vienna Biocenter Austria These authors contributed equally to this work Received 11 January 2007 revised 4 April 2007 accepted 19 April 2007 doi Exposure of the yeast Saccharomyces cerevisiae to weak organic acids such as the food preservatives sorbate benzoate and propionate leads to the pronounced induction of the plasma membrane ATP-binding cassette ABC transporter Pdr12p. This protein mediates efflux of weak acid anions which is essential for stress adaptation. Recently we identified War1p as the dedicated transcriptional regulator required for PDR12 stress induction. Here we report the results from a genetic screen that led to the isolation of two warl alleles encoding mutant variants War1-28p and War1-42p which are unable to support cell growth in the presence of sorbate. DNA sequencing revealed that War1-28 encodes a truncated form of the transcriptional regulator and War1-42 carries three clustered .