tailieunhanh - Báo cáo khoa học: The fabp4 gene of zebrafish (Danio rerio) ) genomic homology with the mammalian FABP4 and divergence from the zebrafish fabp3 in developmental expression

Teleost fishes differ from mammals in their fat deposition and distribution. The gene for adipocyte-type fatty acid-binding protein (A-FABP or FABP4) has not been identified thus far in fishes. We have determined the cDNA sequence and defined the structure of a fatty acid-binding protein gene (designated fabp4) from the zebrafish genome. | ỊFEBS Journal The fabp4 gene of zebrafish Danio rerio - genomic homology with the mammalian FABP4 and divergence from the zebrafish fabp3 in developmental expression Rong-Zong Liu1 Vishal Saxena1 Mukesh K. Sharma1 Christine Thisse2 Bernard Thisse2 Eileen M. Denovan-Wright3 and Jonathan M. Wright1 1 Department of Biology Dalhousie University Halifax Nova Scotia Canada 2 Institut de Genetique et Biologie Moleculaire et Cellulaire Department of DevelopmentalBiology CU de Strasbourg Illkirch France 3 Department of Pharmacology Dalhousie University Halifax Nova Scotia Canada Keywords brain vasculature conserved synteny gene phylogeny linkage mapping whole mount in situ hybridization Correspondence J. M. Wright Department of Biology Dalhousie University Halifax Nova Scotia Canada B3H 4J1 Fax 1 902 494 3736 Tel 1 902 494 6468 E-mail jmwright@ Website http biology2 Received 1 December 2006 revised 15 January 2007 accepted 19 January 2007 doi Teleost fishes differ from mammals in their fat deposition and distribution. The gene for adipocyte-type fatty acid-binding protein A-FABP or FABP4 has not been identified thus far in fishes. We have determined the cDNA sequence and defined the structure of a fatty acid-binding protein gene designated fabp4 from the zebrafish genome. The polypeptide sequence encoded by zebrafish fabp4 showed highest identity to the Had-FABP or H6-FABP from Antarctic fishes and the putative orthologs from other teleost fishes 83-88 . Phylogenetic analysis clustered the zebrafish FABP4 with all Antarctic fish H6-FABPs and putative FABP4s from other fishes in a single clade and then with the mammalian FABP4s in an extended clade. Zebrafish fabp4 was assigned to linkage group 19 at a distinct locus from fabp3. A number of closely linked syntenic genes surrounding the zebrafish fabp4 locus were found to be conserved with human FABP4. The zebrafish fabp4 transcripts showed sequential distribution in the .