tailieunhanh - Báo cáo khoa học: Cyclosporin A-induced oxidative stress is not the consequence of an increase in mitochondrial membrane potential

Cyclosporin A induces closure of the mitochondrial permeability transition pore. We aimed to investigate whether this closure results in concomitant increases in mitochondrial membrane potential (DWm) and the produc-tion of reactive oxygen species. | ỊFEBS Journal Cyclosporin A-induced oxidative stress is not the consequence of an increase in mitochondrial membrane potential Marco van der Toorn1 Henk F. Kauffman2 Margaretha van der Deen3 Dirk-Jan Slebos1 Gerard H. Koeter1 Rijk O. B. Gans1 and Stephan J. L. Bakker1 1 Department of InternalMedicine University MedicalCenter Groningen University of Groningen the Netherlands 2 Groningen University Institute for Drug Exploration University MedicalCenter Groningen University of Groningen the Netherlands 3 Department of MedicalOncology University MedicalCenter Groningen University of Groningen the Netherlands Keywords cyclosporin A mitochondria mitochondrial membrane potential mitochondrial permeability transition reactive oxygen species Correspondence S. J. L. Bakker Department of Internal Medicine University MedicalCenter Groningen PO Box 30001 9700 RB Groningen the Netherlands Fax 31 503 619069 Tel 31 503 613677 E-mail Received 7 December 2006 revised 6 April 2007 accepted 11 April 2007 doi Cyclosporin A induces closure of the mitochondrial permeability transition pore. We aimed to investigate whether this closure results in concomitant increases in mitochondrial membrane potential AW j and the production of reactive oxygen species. Fluorescent probes were used to assess A Wm JC-1 5 5 6 6 -tetrachloro-1 1 3 3 -tetraethyl-benzimidazolyl-carbo-cyanine iodide reactive oxygen species DCF 5- and 6 -chloromethyl-2 7 -dichlorodihydrofluorescein diacetate acetyl ester and Ca2 Fluo-3 glycine N- 4- 6- acetyloxy methoxy -2 7-dichloro-3-oxo-3H-xanthen-9-yl -2- 2- 2- bis 2- acetyloxy methoxy -2-oxyethyl amino -5-methylphenoxy eth-oxy phenyl -N- 2- acetyloxy methoxy -2-oxyethyl - acetyloxy methyl ester in human kidney cells HK-2 cells and in a line of human small cell carcinoma cells GLC4 cells because these do not express cyclosporin A-sensitive P-glycoprotein. We used transfected GLC4 cells expressing P-glycoprotein as .