tailieunhanh - Báo cáo khoa học: Metabolic pathway that produces essential fatty acids from polymethylene-interrupted polyunsaturated fatty acids in animal cells

Sciadonic acid (20:3D-5,11,14) and juniperonic acid (20:4 D-5,11,14,17) are polyunsaturated fatty acids (PUFAs) that lack the D-8 double bond of arachidonic acid (20:4 D-5,8,11,14) and eicosapentaenoic acid (20:5 D-5,8,11,14,17), respectively. | ễFEBS Journal Metabolic pathway that produces essential fatty acids from polymethylene-interrupted polyunsaturated fatty acids in animal cells Tamotsu Tanaka1 Jun-ichi Morishige1 Dai Iwawaki1 Terumi Fukuhara1 Naomi Hamamura1 Kaoru Hirano1 Takashi Osumi2 and Kiyoshi Satouchi1 1 Department of Applied BiologicalScience Fukuyama University Hiroshima Japan 2 Graduate Schoolof Life Sciences University of Hyogo Japan Keywords essential fatty acids peroxisomal b-oxidation polymethylene-interrupted polyunsaturated fatty acids polyunsaturated fatty acid remodeling Correspondence T. Tanaka Department of Applied Biological Science Fukuyama University Higashimura Fukuyama Hiroshima 729-0292 Japan Fax 81 84 9362459 Tel 81 84 9362111 E-mail tamot@ Received 22 January 2007 revised 9 March 2007 accepted 23 March 2007 doi Sciadonic acid 20 3 D-5 11 14 and juniperonic acid 20 4 D-5 11 14 17 are polyunsaturated fatty acids PUFAs that lack the D-8 double bond of arachidonic acid 20 4 D-5 8 11 14 and eicosapentaenoic acid 20 5 D-5 8 11 14 17 respectively. Here we demonstrate that these conifer oil-derived PUFAs are metabolized to essential fatty acids in animal cells. When Swiss 3T3 cells were cultured with sciadonic acid linoleic acid 18 2 D-9 12 accumulated in the cells to an extent dependent on the concentration of sciadonic acid. At the same time a small amount of 16 2 D-7 10 appeared in the cellular lipids. Both 16 2 D-7 10 and linoleic acid accumulated in sciadonic acid-supplemented CHO cells but not in peroxisome-deficient CHO cells. We confirmed that 16 2 D-7 10 was effectively elongated to linoleic acid in rat liver microsomes. These results indicate that sciadonic acid was partially degraded to 16 2 D-7 10 by two cycles of b-oxidation in peroxisomes then elongated to linoleic acid in microsomes. Supplementation of Swiss 3T3 cells with juniperonic acid an n-3 analogue of sciadonic acid induced accumulation of a-linolenic .