tailieunhanh - Báo cáo khoa học: Enzymes that hydrolyze adenine nucleotides of patients with hypercholesterolemia and inflammatory processes

The activity of NTPDase (EC , apyrase, CD39) was verified in plate-lets from patients with increasing cholesterol levels. A possible association between cholesterol levels and inflammatory markers, such as oxidized low-density lipoprotein, highly sensitive C-reactive protein and oxidized low-density lipoprotein autoantibodies, was also investigated. | ỊFEBS Journal Enzymes that hydrolyze adenine nucleotides of patients with hypercholesterolemia and inflammatory processes Marta Medeiros Frescura Duarte1 Vania L. Loro1 Joao B. T. Rocha1 Daniela B. R. Leal2 Andreza F. de Bem1 Araceli Dorneles1 Vera M. Morsch1 and Maria R. C. Schetinger1 1 Departamento de Quimica Centro de Ciencias Naturais e Exatas Programa de Pos-Graduacao em Bioquimica Toxicologica Universidade Federalde Santa Maria Brazil 2 HospitalUniversitario de Santa Maria Santa Maria RS Brazil Keywords cholesterol highly sensitive C-reactive protein NTPDase oxidized low-density lipoprotein oxidized low-density lipoprotein autoantibodies Correspondence M. R. C. Schetinger Departamento de Quimica Centro de Ciencias Naturais e Exatas Universidade Federalde Santa Maria Av. Roraima 1000 97105-900 Santa Maria RS Brazil Fax 55 5532 208978 Tel 55 5532 208665 E-mail mariaschetinger@ Received 18 August 2006 revised 29 January 2007 accepted 19 March 2007 doi The activity of NTPDase EC apyrase CD39 was verified in platelets from patients with increasing cholesterol levels. A possible association between cholesterol levels and inflammatory markers such as oxidized low-density lipoprotein highly sensitive C-reactive protein and oxidized low-density lipoprotein autoantibodies was also investigated. Lipid peroxidation was estimated by measurement of thiobarbituric acid reactive substances in serum. The following groups were studied group I 150 mg dL-1 cholesterol group II 151-200 mg dL-1 cholesterol group -III 201-250 mg dL-1 cholesterol and group IV 251 mg dL-1 cholesterol. The results demonstrated that both ATP hydrolysis and ADP hydrolysis were enhanced as a function of cholesterol level. Low-density lipoprotein levels increased concomitantly with total cholesterol levels. Triglyceride levels were increased in the groups with total cholesterol above 251 mg dL-1. Oxidized low-density lipoprotein levels were elevated in .

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