tailieunhanh - Báo cáo khoa học: Neuroglobin and cytoglobin expression in mice Evidence for a correlation with reactive oxygen species scavenging

Although essentially unknown, several functions are hypothesized for neu-roglobin and cytoglobin, two new members of the globin family. In this article, we try to shed more light on their possible roles in hypoxia and detoxification of reactive oxygen speciesin vivo. Thijs | ễFEBS Journal Neuroglobin and cytoglobin expression in mice Evidence for a correlation with reactive oxygen species scavenging Elke Fordel Liesbet Thijs Luc Moens and Sylvia Dewilde Department of BiomedicalSciences University of Antwerp Belgium Keywords cytoglobin eyes hypoxia neuroglobin reactive oxygen species Correspondence S. Dewilde Department of Biomedical Sciences University of Antwerp Universiteitsplein 1 B-2610 Antwerp Belgium Fax 32 3 8202248 Tel 32 3 8202392 E-mail Received 29 September 2006 revised 7 December 2006 accepted 4 January 2007 doi Although essentially unknown several functions are hypothesized for neuroglobin and cytoglobin two new members of the globin family. In this article we try to shed more light on their possible roles in hypoxia and detoxification of reactive oxygen species in vivo. The relative transcriptional changes of neuroglobin and cytoglobin in a situation of chronic hypoxia in mice were examined using real-time quantitative PCR. The kinetics of the hypoxic expression of neuroglobin brain eyes and cytoglobin brain eyes liver heart skeletal muscle is organ-specific. Moreover reactive oxygen species production is higher in liver than in the other tissues. In eyes the negative correlation after reoxygenation between neuroglobin protein level and H2O2 concentration is a first proof of a reactive oxygen speciesscavenging function for neuroglobin. In addition apoptotic cell death after hypoxia is for the first time demonstrated in heart and liver. Both hypoxia lack of O2 relative to metabolic needs and reoxygenation reintroduction of O2 to hypoxic tissue are important in human pathophysiology. Prominent examples of tissue hypoxia that predispose to injury during reoxygenation include myocardial ischemia stroke and transplantation of organs. Understanding the role of reactive oxygen species ROS in reoxygenation injury has the potential to lead to therapies that could improve .