tailieunhanh - Báo cáo khoa học: Multiple promoters regulate tissue-specific alternative splicing of the human kallikrein gene, KLK11⁄hippostasin

The human kallikrein (KLK) family consists of 15 genes located on human chromosome . KLK11⁄hippostasin is a member of the kallikrein family and is expressed in various tissues. Two types of KLK11 isoforms, isoform 1 and isoform 2, have been predicted from cDNA sequences. Iso-form 1 has been isolated from human hippocampus, whereas isoform 2 has been isolated from prostate. | ễFEBS Journal Multiple promoters regulate tissue-specific alternative splicing of the human kallikrein gene KLK11 hippostasin 1 2 -3 -33 Shinichi Mitsui Terukazu Nakamura Akira Okui Katsuya Kominami Hidetoshi Uemura and Nozomi Yamaguchi1 1 Department of Cell Biology Research Institute for Geriatrics Kyoto PrefecturalUniversity of Medicine Kyoto Japan 2 Department of Urology Kyoto PrefecturalUniversity of Medicine Kyoto Japan 3 Research and Development Center Fuso PharmaceuticalIndustries Ltd Osaka Japan Keywords hippocampus oligo cap RACE prostate serine protease Correspondence N. Yamaguchi Cell Biology and Protein Engineering EnvironmentalSystems Science Doshisha University Kyontanabe Kyoto 610-0394 Japan Fax Tel 81 774065 6676 E-mail nyamaguc@ Present address Department of Neurobiology and Anatomy Kochi Medical School Okou Nankoku 7838505 Japan Database The nucleotide sequence reported in this paper has been entered in the DDBJ GeriBank EMBL databases under accession number AB259014 Received 27 January 2006 revised 5 June 2006 accepted 12 June 2006 doi The human kallikrein KLK family consists of 15 genes located on human chromosome . KLK11 hippostasin is a member of the kallikrein family and is expressed in various tissues. Two types of KLK11 isoforms isoform 1 and isoform 2 have been predicted from cDNA sequences. Isoform 1 has been isolated from human hippocampus whereas isoform 2 has been isolated from prostate. However the regulation and characteristics of these isoforms are unknown. We identified the first three exons 1a 1b and 1c by determining their transcription initiation sites. Exon 1b contained the initiation codon of isoform 2 and noncoding exons 1a and 1c contributed to isoform 1 mRNA. The dual luciferase promoter assay revealed three promoter regions corresponding to the first exon of each isoform. Reverse transcription and PCR showed that exon 1a was expressed in the hippocampus thalamus .

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