tailieunhanh - Báo cáo khoa học: Identification of a cowpea c-thionin with bactericidal activity

Antimicrobial peptides are an abundant group of proteinaceous com-pounds widely produced in the plant kingdom. Among them, thec-thionin family, also known as plant defensins, represents one typical family and comprises low molecular mass cysteine-rich proteins, usually cationic and distributed in different plant tissues. | ềFEBS Journal Identification of a cowpea c-thionin with bactericidal activity Octavio L. Franco1 Andre M. Murad1 Jose R. Leite2 Paulo A. M. Mendes1 Maura V. Prates2 and Carlos Bloch Jr2 1 Centro de Analise Proteômicas e Bioquímicas Pós-Graduaẹão em Ciências Genômicas e Biotecnologia UCB Brasilia-DF Brazil 2 Laboratorio de Espectrometria de Massa Embrapa Recursos Geneticos e Biotecnologia Brasilia-DF Brazil Keywords bactericidal defensin y-thionin germination Vigna unguiculata Correspondence O. L. Franco Universidade Catolica de Brasilia Pos-graduacao em Ciencias Genomicas e Biotecnologia SGAN 916 Av. W5 Campus II Modulo C Sala 222 Brasilia-DF Fax 55 61 33474797 Tel 55 61 34487220 E-mail ocfranco@ Received 27 March 2006 revised 23 May 2006 accepted 2 June 2006 doi Antimicrobial peptides are an abundant group of proteinaceous compounds widely produced in the plant kingdom. Among them the y-thionin family also known as plant defensins represents one typical family and comprises low molecular mass cysteine-rich proteins usually cationic and distributed in different plant tissues. Here we report the purification and characterization of a novel y-thionin from cowpea seeds Vigna ungu-iculata named Cp-thionin II with bactericidal activity against Grampositive and Gram-negative bacteria. Once the primary structure was elucidated molecular modelling experiments were used to investigate the multimerization and mechanism of action of plant y-thionins. Furthermore Cp-thionin II was also localized in different tissues in cowpea seedlings during germination in contrasting conditions to better understand the plant protection processes. The use of plant defensins in the construction of transgenic plants and also in the production of novel drugs with activity against human pathogens is discussed. Plants are able to produce compounds that act as natural defences against pests and pathogens 1 2 . Among known plant protection compounds are the

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