tailieunhanh - Báo cáo khoa học: Observation of a chaotic multioscillatory metabolic attractor by real-time monitoring of a yeast continuous culture

We monitored a continuous culture of the yeastSaccharomyces cerevisiae by membrane-inlet mass spectrometry. This technique allows very rapid simultaneous measurements (one point every 12 s) of several dissolved gases. During our experiment, the culture exhibited a multioscillatory mode in which the dissolved oxygen and carbon dioxide records displayed period-icities of 13 h, 36 min and 4 min. | ỊFEBS Journal Observation of a chaotic multioscillatory metabolic attractor by real-time monitoring of a yeast continuous culture Marc R. Roussel1 2 and David Lloyd1 1 Microbiology Group Cardiff Schoolof Biosciences Cardiff University UK 2 Department of Chemistry and Biochemistry University of Lethbridge Canada Keywords biochemical oscillations chaos continuous culture yeast Correspondence M. R. Roussel Department of Chemistry and Biochemistry University of Lethbridge Lethbridge Alberta T1K 3M4 Canada Fax 1 403 329 2057 Tel 1 403 329 2326 E-mail roussel@ Website http roussel Received 7 November 2006 revised 6 December 2006 accepted 14 December 2006 We monitored a continuous culture of the yeast Saccharomyces cerevisiae by membrane-inlet mass spectrometry. This technique allows very rapid simultaneous measurements one point every 12 s of several dissolved gases. During our experiment the culture exhibited a multioscillatory mode in which the dissolved oxygen and carbon dioxide records displayed periodicities of 13 h 36 min and 4 min. The 36- and 4-min modes were not visible at all times but returned at regular intervals during the 13-h cycle. The 4-min mode which has not previously been described in continuous culture can also be seen when the culture displays simpler oscillatory behavior. The data can be used to visualize a metabolic attractor of this system . the set of dissolved gas concentrations which are consistent with the multioscillatory state. Computation of the leading Lyapunov exponent reveals the dynamics on this attractor to be chaotic. doi Organisms carry out processes necessary for the maintenance of life on many time scales 1 . Not all possible cellular processes are compatible so either temporal or spatial separation of activity is required 2 . Temporal coordination is provided by biological clocks such as the circadian 2 3 and circahoralian with periods T of 1 h 4-9 both of which are .

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