tailieunhanh - Báo cáo khoa học: Fermentative lifestyle in yeasts belonging to the Saccharomyces complex

The yeastSaccharomyces cerevisiaeis characterized by its ability to: (a) degrade glucose or fructose to ethanol, even in the presence of oxygen (Crabtree effect); (b) grow in the absence of oxygen; and (c) generate respir-atory-deficient mitochondrial mutants, so-called petites. | ễFEBS Journal Fermentative lifestyle in yeasts belonging to the Saccharomyces complex Annamaria Merico1 Pavol Sulo2 Jure Piskur3 and Concetta Compagno1 1 Dipartimento di Scienze Biomolecolari e Biotecnologie University degli Studi di Milano Milan Italy 2 Department of Biochemistry Faculty of NaturalSciences Comenius University Bratislava Slovakia 3 Department of Celland Organism Biology Lund University Sweden Keywords evolution fermentation petite mutants redox metabolism respiration Correspondence C. Compagno Dipartimento di Scienze Biomolecolari e Biotecnologie Universita degli Studi di Milano via Celoria 26 20133 Milan Italy Fax 39 02503 14912 E-mail Received 9 October 2006 revised 24 November 2006 accepted 11 December 2006 doi The yeast Saccharomyces cerevisiae is characterized by its ability to a degrade glucose or fructose to ethanol even in the presence of oxygen Crabtree effect b grow in the absence of oxygen and c generate respiratory-deficient mitochondrial mutants so-called petites. How unique are these properties among yeasts in the Saccharomyces clade and what is their origin Recent progress in genome sequencing has elucidated the phylogenetic relationships among yeasts in the Saccharomyces complex providing a framework for the understanding of the evolutionary history of several modern traits. In this study we analyzed over 40 yeasts that reflect over 150 million years of evolutionary history for their ability to ferment grow in the absence of oxygen and generate petites. A great majority of isolates exhibited good fermentation ability suggesting that this trait could already be an intrinsic property of the progenitor yeast. We found that lineages that underwent the whole-genome duplication in general exhibit a fermentative lifestyle the Crabtree effect and the ability to grow without oxygen and can generate stable petite mutants. Some of the pre-genome duplication lineages also exhibit some