tailieunhanh - Báo cáo khoa học: Characterization of natural vasostatin-containing peptides in rat heart

Chromogranin A (CGA) is a protein that is stored and released together with neurotransmitters and hormones in the nervous, endocrine and diffuse neuroendocrine systems. As human vasostatins I and II [CGA(1–76) and CGA(1–113), respectively] have been reported to affect vessel motility and exert concentration-dependent cardiosuppressive effects on isolated whole heart preparations of eel, | ềFEBS Journal Characterization of natural vasostatin-containing peptides in rat heart Elise Glattard1 Tommaso Angelone2 Jean-Marc Strub3 Angelo Corti4 Dominique Aunis1 Bruno Tota2 Marie-Hélène Metz-Boutigue1 and Yannick Goumon1 1 Inserm U575 Physiopathologie du Systeme Nerveux Strasbourg France 2 Laboratory of Cardiovascular Physiology Department of Cell Biology University of Calabria Arcavacata di Rende Italy 3 CNRS UMR 7512 Laboratoire de Spectrometrie de Masse BioOrganique Strasbourg France 4 Department of Biologicaland TechnologicalResearch San Raffaele H Scientific Institute Milan Italy Keywords chromogranin A heart post-translational modifications rat vasostatin Correspondence Y. Goumon Inserm U575 5 rue Blaise Pascal F-67084 Strasbourg Cedex France Fax 33 3 88 60 08 06 Tel 33 3 88 45 67 24 E-mail goumon@ Received 17 February 2006 revised 18 May 2006 accepted 24 May 2006 doi Chromogranin A CGA is a protein that is stored and released together with neurotransmitters and hormones in the nervous endocrine and diffuse neuroendocrine systems. As human vasostatins I and II CGA 1-76 and CGA 1-113 respectively have been reported to affect vessel motility and exert concentration-dependent cardiosuppressive effects on isolated whole heart preparations of eel frog and rat . negative inotropism and anti-adrenergic activity we investigated the presence of vasostatin-containing peptides in rat heart. Rat heart extracts were purified by RP-HPLC and the resulting fractions analyzed for the presence of CGA N-terminal fragments using dot-blot analysis. CGA-immunoreactive fractions were submitted to western blot and MS analysis using the TOF TOF technique. Four endogenous N-terminal CGA-derived peptides CGA 4-113 CGA 1-124 CGA 1-135 and CGA 1-199 containing the vasostatin sequence were characterized. The following post-translational modifications of these fragments were identified phosphorylation at Ser96 O-glycosylation