tailieunhanh - Báo cáo khoa học: Differential regulation of fatty acid amide hydrolase promoter in human immune cells and neuronal cells by leptin and progesterone

We have shown recently that in human T lymphocytes, leptin stimulates activity and expression of the endocan-nabinoid-degrading enzyme fatty acid amide hydrolase (FAAH), through STAT3 (signal transducer and activator of transcription 3) and its CRE (cAMP response element)-like transcriptional target in the FAAH promoter [Maccar-rone, M., Di Rienzo, M., Finazzi-Agro`,A., &Rossi,A. (2003) J. Biol. Chem. 278, 13318–13324]. We have also shown that progesterone, alone or additively with leptin, up-regulates theFAAHgene in human T-cells, through the Ikaros transcription factor [Maccarrone, M., Bari, M., Di Rienzo,. | Eur. J. Biochem. 271 4666-4676 2004 FEBS 2004 doi Differential regulation of fatty acid amide hydrolase promoter in human immune cells and neuronal cells by leptin and progesterone Mauro Maccarrone1 2 Valeria Gasperi3 Filomena Fezza1 Alessandro Finazzi-Agro3 and Antonello Rossi3 1 Department of Biomedical Sciences University of Teramo Italy 2IRCCS C. Mondino Mondino-Tor Vergata-Santa Lucia Center for Experimental Neurobiology Rome Italy 3Department of Experimental Medicine and Biochemical Sciences University of Rome Tor Vergata Rome Italy We have shown recently that in human T lymphocytes leptin stimulates activity and expression of the endocannabinoid-degrading enzyme fatty acid amide hydrolase FAAH through STAT3 signal transducer and activator of transcription 3 and its CRE cAMP response element -like transcriptional target in the FAAH promoter Maccar-rone M. Di Rienzo M. Finazzi-Agro A. Rossi A. 2003 J. Biol. Chem. 278 13318-13324 . We have also shown that progesterone alone or additively with leptin up-regulates the FAAH gene in human T-cells through the Ikaros transcription factor Maccarrone M. Bari M. Di Rienzo M. Finazzi-Agro A. Rossi A. 2003 J. Biol. Chem. 278 32726-32732 . Here we extend these observations to immortalized human lymphoma U937 cells where stimulation of FAAH by leptin up to w 300 of the controls involves binding to a leptin receptor Kd nM Bmax 382 5 fmol-mg protein-1 appar ent molecular mass of w 110 kDa and stimulation by progesterone involves an intracellular receptor of w 120 kDa. Unlike FAAH the other proteins of the endocannabinoid system are not modulated by the two hormones. Interestingly human neuroblastoma CHP100 cells also have a leptin receptor w 110 kDa Kd nM Bmax 339 8 fmolmg protein-1 a progesterone receptor w 120 kDa STAT3 and Ikaros yet their FAAH is not activated by leptin or progesterone. These data corroborated by transient expression and electrophoretic mobility-shift .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• medicine
• cell proliferation
• oxidation
• Selective Oxidation
• Gold Nanoparticles
• Aerobic Oxidation
• Oxidation Catalysts
• Crystalline Materials
• Molecular Oxygen
• Nuclear engineering and technology
• The recycling of nuclear graphite after micro oxidation
• Nuclear graphite after micro oxidation
• Whether ground micro oxidation graphite
• The oxidized graphite particles
• The recycling of nuclear graphite
• Dietary fructose
• Fatty acid oxidation
• Hepatic steatosis
• Fatty acid β oxidation
• Lipid oxidation
• Meal extracts
• Refined soybean oil
• Total oxidation value
• Meal extracts on retarding lipid oxidation
• Environmental advances
• Volatile organic compounds
• Ozone oxidation technique
• Catalytic ozone oxidation
• Ozone oxidation
• breast cancer
• disorders
• Fatty acid
• Vietnam Journal of Science and Technology
• Masterbatch containing anti oxidation additive
• Anti oxidation master batch
• Melt flow index
• Tensile strength and elongation
• Vietnam Journal of Chemistry
• Mg Al MoO4 layered double hydroxides
• Catalysts for the oxidation of styrene
• The oxidation of styrene
• The synthesized solids showed the layered hydroxide structure
• Effects of ascorbic acid treatment
• Packaging method on lipid oxidation
• Frozen storage
• Ascorbic acid
• Vacuum packaging
• Lipid oxidation
• CO oxidation
• Manganese oxides
• Binary catalysts
• Formic acid oxidation
• Novel binary catalysts
• Thermal spray
• NiCr Al duplex coating
• Oxidation resistance
• Carbon steel substrate
• High temperature oxidation resistant
• High temperature working parts
• High temperature steam oxidation kinetics
• High temperature oxidation
• Weight gain measurements
• E110 cladding tubes material
• Copper oxide
• Thin films
• Thermal oxidation
• Annealing temperature
• Thermal oxidation method
• X ray diffraction measurement
• Journal of Chemistry
• Aftertreatment systems
• Diesel oxidation catalyst
• Bimetallic catalyst
• Commercial monolith
• Journal of Science and Technology
• Plasma electrolytic oxidation coating
• 6061 Al alloy
• Electrolyte without alkali ions
• Plasma electrolytic oxidation
• Porous nickel
• Cast nickel
• Duplex macrostructure
• Inward migration
• Linear kinetics
• Redox process
• Oxidation reduction reactions
• Oxidation reduction reactions in geochemistry
• Extremely reducing or oxidizing conditions
• Reduction potentials in geochemistry
• Benzyl alcohol oxidation
• Peripherally 1 2 dinaphthyl ethanediol
• Phthalocyanine complexes
• UV Vis light absorption
• Catalytic oxidation
• polyamine
• oxidase K300M
• Crystal structure
• bacteria
• scientific research
• biochemistry
• hemoglobin
• biopreservation
• cytoplasm
• chemical reaction
• tumor cells