tailieunhanh - Báo cáo khoa học: Hierarchical subfunctionalization of fabp1a, fabp1b and fabp10 tissue-specific expression may account for retention of these duplicated genes in the zebrafish (Danio rerio) genome

Fatty acid-binding protein type 1 (FABP1), commonly termed liver-type fatty acid-binding protein (L-FABP), is encoded by a single gene in mam-mals. We cloned and sequenced cDNAs for two distinct FABP1s in zebra-fish coded by genes designatedfabp1a andfabp1b. The zebrafish proteins, FABP1a and FABP1b, show highest sequence identity and similarity to the human protein FABP1. | iFEBS Journal Hierarchical subfunctionalization of fabpla fabplb and fabp10 tissue-specific expression may account for retention of these duplicated genes in the zebrafish Danio rerio genome Mukesh K. Sharma1 Rong-Zong Liu1 Christine Thisse2 Bernard Thisse2 Eileen M. Denovan-Wright3 and Jonathan M. Wright1 1 Department of Biology Dalhousie University Halifax NS Canada 2 Institut de Genetique et de Biologie Moleculaire et Cellulaire Department of DevelopmentalBiology Illkirch France 3 Department of Pharmacology Dalhousie University Halifax NS Canada Keywords embryonic development FABP1 gene duplication linkage mapping subfunctionalization Correspondence J. M. Wright Department of Biology Dalhousie University Halifax Nova Scotia Canada B3H 4J1 Fax 1 902 494 3736 Tel 1 902 494 6468 E-mail jmwright@ Database The following sequences have been submitted to the GenBank database under accession numbers DQ062095 fabpla cDNA DQ062096 fabplb cDNA long transcript variant and DQ474062 fabplb cDNA short transcript variant . Received 30 January 2006 revised 10 April 2006 accepted 18 May 2006 doi Fatty acid-binding protein type 1 FABP1 commonly termed liver-type fatty acid-binding protein L-FABP is encoded by a single gene in mammals. We cloned and sequenced cDNAs for two distinct FABP1s in zebrafish coded by genes designated fabpla and fabplb. The zebrafish proteins FABP1a and FABP1b show highest sequence identity and similarity to the human protein FABP1. Zebrafish fabpla and fabplb genes were assigned to linkage groups 5 and 8 respectively. Both linkage groups show conserved syntenies to a segment of mouse chromosome 6 rat chromosome 4 and human chromosome 2 harboring the FABP1 locus. Phylogenetic analysis further suggests that zebrafish fabpla and fabplb genes are orthologs of mammalian FABPl and most likely arose by a whole-genome duplication event in the ray-finned fish lineage estimated to have occurred 200-450 million years ago. The .