tailieunhanh - Báo cáo khoa học: Kinetics of intra- and intermolecular zymogen activation with formation of an enzyme–zymogen complex

A mathematical description was made of an autocatalytic zymogen activa-tion mechanism involving both intra- and intermolecular routes. The reversible formation of an active intermediary enzyme–zymogen complex was included in the intermolecular activation route, thus allowing a Micha-elis–Menten constant to be defined for the activation of the zymogen towards the active enzyme. | ềFEBS Journal Kinetics of intra- and intermolecular zymogen activation with formation of an enzyme-zymogen complex Matilde Esther Fuentes Ramon Varon Manuela Garcia-Moreno and Edelmira Valero Grupo de Modelizacion en Bioquimica Departamento de Quimica-Fisica Escuela Politecnica Superior de Albacete Universidad de Castilla-La Mancha Albacete Spain Keywords autocatalysis enzyme kinetics pepsin pepsinogen zymogen Correspondence E. Valero Grupo de Modelizacion en Bioquimica Departamento de Quimica-Fisica Escuela Politecnica Superior de Albacete Universidad de Castilla-La Mancha Avda. Espana s n Campus Universitario E-02071 Albacete Spain Fax 34 967 59 92 24 Tel 34 967 59 92 00 E-mail Note The mathematical model described here has been submitted to the Online Cellular Systems Modelling Database and can be accessed free of charge at http database fuentes A mathematical description was made of an autocatalytic zymogen activation mechanism involving both intra- and intermolecular routes. The reversible formation of an active intermediary enzyme-zymogen complex was included in the intermolecular activation route thus allowing a Micha-elis-Menten constant to be defined for the activation of the zymogen towards the active enzyme. Time-concentration equations describing the evolution of the species involved in the system were obtained. In addition we have derived the corresponding kinetic equations for particular cases of the general model studied. Experimental design and kinetic data analysis procedures to evaluate the kinetic parameters based on the derived kinetic equations are suggested. The validity of the results obtained were checked by using simulated progress curves of the species involved. The model is generally good enough to be applied to the experimental kinetic study of the activation of different zymogens of physiological interest. The system is illustrated by following the transformation kinetics of .

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