tailieunhanh - Báo cáo khoa học: Akt-dependent phosphorylation negatively regulates the transcriptional activity of dHAND by inhibiting the DNA binding activity

Tham khảo luận văn - đề án 'báo cáo khoa học: akt-dependent phosphorylation negatively regulates the transcriptional activity of dhand by inhibiting the dna binding activity', luận văn - báo cáo, báo cáo khoa học phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Eur. J. Biochem. 271 3330-3339 2004 FEBS 2004 doi Akt-dependent phosphorylation negatively regulates the transcriptional activity of dHAND by inhibiting the DNA binding activity Masao Murakami1 2 Keiichiro Kataoka1 Shigetomo Fukuhara1 Osamu Nakagawa2 and Hiroki Kurihara1 3 Division of Integrative Cell Biology Department of Embryogenesis Institute of Molecular Embryology and Genetics Kumamoto University Japan 2Department of Molecular Biology The University of Texas Southwestern Medical Center at Dallas TX USA 3Department of Physiological Chemistry and Metabolism The University of Tokyo Graduate School of Medicine Japan HAND2 dHAND is a basic helix-loop-helix transcription factor expressed in the heart and neural crest derivatives during embryogenesis. Although dHAND is essential for branchial arch cardiovascular and limb development its target genes have not been identified. The regulatory mechanisms of dHAND function also remain relatively unknown. Here we report that Akt PKB a serine threonine protein kinase involved in cell survival growth and differentiation phosphorylates dHAND and inhibits dHAND-mediated transcription. AU5-dHAND expressed in 293T cells became phosphorylated possibly at its Akt phosphorylation motif in the absence of kinase inhibitors whereas the phosphatidylinositol 3-kinase inhibitor wort-mannin and the Akt inhibitor NL-71-101 but not the p70 S6 kinase inhibitor rapamycin significantly reduced dHAND phosphorylation. Coexpression of HA-Akt augmented dHAND phosphorylation at multiple serine and threonine residues mainly located in the bHLH domain and as a result decreased the transcriptional activity of dHAND. Consistently alanine mutation mimicking the nonphosphorylation state abolished the inhibitory effect of Akt on dHAND whereas aspartate mutation mimicking the phosphorylation state resulted in a loss of dHAND transcriptional activity. These changes in dHAND transcriptional activity were in parallel with .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• breast cancer
• medicine
• chemical reaction
• Elements of Chemical Reaction Engineering
• Chemical Reaction Engineering
• Hóa học và ứng dụng
• Kỹ thuật hóa học
• Phản ứng hóa học
• Kỹ thuật phản ứng hóa học
• The chemical vapor deposition condition
• The electrochemically catalytic efficiency
• Hydrogen evolution reaction in MoS2 nanoparticles
• Hydrogen evolution reaction
• Metal organic chemical vapor deposition
• Reaction Kinetics
• Heterogeneous Catalysis
• Elementary Reactions
• Microkinetic Analysis
• Nonisothermal Reactors
• Catalytic Reactions
• Quantum chemical modeling mechanism
• Semiempirical method RM1
• Quantum chemical study
• Half chair shape
• Diels Alder reaction
• Chemical reaction engirneering
• Hoá phân tích
• Giải pháp phản ứng hóa học
• Radiation on MHD casson fluid flow
• Chemical reaction effects on MHD casson fluid flow
• Porous medium with suction injection
• The occurrence of suction/injection
• The central PDEs
• Radiation of MHD visco elastic fluid flow over
• Chemical reaction on heat of MHD visco elastic
• Mass transfer of MHD visco elastic fluid flow
• Exponentially stretching sheet through porous medium
• Radiation on the boundary layer MHD casson fluid
• Chemical reaction effects on the boundary layer MHD casson fluid
• Vertical plate embedded in the porous medium
• Casson fluid flow free convection boundary layer fl
• Extreme learning machine
• Artificial chemical reaction optimization algorithm
• Single hidden layer feed forward neural network
• Learning algorithm
• Chemical reaction kinetic
• Adsorption kinetics
• Pseudo first order model
• Pseudo second order model
• General kinetic model
• Dried Probiotics
• Industrial Processes
• Membrane Separation
• Wastewater
• Maillard Reaction
• Pros and Cons
• Chemical kinetics
• Reactant concentration
• Reaction rates and stoichiometry
• Action of catalysts
• Surface area
• Chemical kinetics
• Introduction to general
• Chemical bonds
• Chemical reactions
• Reaction rates
• Chemical equilibrium
• Nuclear chemistry
• Nuclear engineering and technology
• Correlation between rare earth elements
• The chemical interactions of HT9 cladding
• FueleCladding Chemical Interaction
• Higher reaction thickness
• Summary of Chemical PhD Thesis
• Theoretical and Physical Chemistry
• CO hydrogenation reaction
• Ni Cu bimetallic catalyst system
• Density functional method
• Chemical method
• Lecture General Chemistry 1
• Bài giảng Hóa học đại cương 1
• Balance chemical equations
• Calculate the percent yield of a reaction
• Chemical formulas
• Chemical equations
• organic chemistry
• hóa hữu cơ
• công nghệ hóa học
• Chemical Engineering
• common reaction mechanisms
• Chromatography in the petroleum industry
• oxidation
• tumor cells
• BMC Bioinformatics
• Flux balance analysis
• Linear programming
• Design of metabolic network
• Constraint based model
• Growth rate
• Production rate
• Smaller reaction network
• Luận án Tiến sĩ
• Physical chemistry
• Fabbricated electrocatalysts
• Oxygen evolution reaction
• Proton exchange membrane
• cytotoxicity
• Atomic nucleus
• electrons
• Chemical compounds
• Electrochemistry