tailieunhanh - Báo cáo khoa học: Protein transport into canine pancreatic microsomes A quantitative approach

Transport of presecretory proteins into the mammalian rough endoplasmic reticulum involves a protein translocase that comprises the integral membrane proteins Sec61ap, Sec61bp, and Sec61cp as core components. Electron microscopic analysis of protein translocase in rough micro-somal membranes suggested that between three and four heterotrimeric Sec61p complexes form the central unit of protein translocase. Here we analyzed the stoichiometry of heterotrimericSec61pcomplexespresent incotranslationally active protein translocases of canine pancreatic microsomes and various other lumenal and membrane components believed to be subunits of protein translocase and to be involved in covalentmodifications | Eur. J. Biochem. 271 3200-3207 2004 FEBS 2004 doi Protein transport into canine pancreatic microsomes A quantitative approach Silvia Guth Christian Volzing Anika Muller Martin Jung and Richard Zimmermann Medizinische Biochemie und Molekularbiologie Universitat des Saarlandes Homburg Germany Transport of presecretory proteins into the mammalian rough endoplasmic reticulum involves a protein translocase that comprises the integral membrane proteins Sec61ap Sec61bp and Sec61yp as core components. Electron microscopic analysis of protein translocase in rough microsomal membranes suggested that between three and four heterotrimeric Sec61p complexes form the central unit of protein translocase. Here we analyzed the stoichiometry of heterotrimeric Sec61p complexes present in cotranslationally active protein translocases of canine pancreatic microsomes and various other lumenal and membrane components believed to be subunits of protein translocase and to be involved in covalent modifications. Based on these numbers the capacity for cotranslational transport was estimated for the endoplasmic reticulum of the human pancreas. Keywords endoplasmic reticulum mammalian microsomes protein secretion protein transport pancreas. Transport of presecretory proteins into mammalian rough microsomes involves cleavable signal peptides at the N-terminus of the precursor proteins and a protein translocase in the microsomal membrane 1 . Typically transport occurs as a sequence of three consecutive steps namely a specific membrane association of the precursor protein also termed targeting b membrane insertion and c completion of translocation. Specific membrane association of precursors in cotranslational transport involves two ribonu-cleoparticles - the ribosome 2 and the signal recognition particle SRP 3 - as well as their receptors on the endoplasmic reticulum ER surface the SRP and ribosome receptors 4 5 . Protein translocase a mediates both membrane .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• Antimicrobial
• cell structure
• genetics
• Biological information
• Lecture Genetics
• Human genetics
• Organelle genetics
• Bacterial genomes
• Non Mendelian inheritance
• Somatic mutation
• Genetics of cancer
• Cell division
• Model organisms
• Developmental genetics
• BRS Genetics
• Chromosomal morphology methods
• Cytogenetic disorders
• Genetics of metabolism
• Genetics of hemoglobinopathies
• Genetics of bleeding disorders
• Quantitative genetics
• Population genetics
• Evolutionary genetics
• Cancer genetics
• The genetic code
• Ebook Principles of Genetics
• Principles of Genetics
• Principles of Genetics (Sixth edition)
• The science of genetics
• Cellular reproduction
• Testing genetic hypotheses
• The techniques of molecular genetics
• Transposable genetic elements
• Inheritance of complex traits
• Applications of molecular genetics
• Genetics in medicine
• Birth defects
• Cancer genetics and genomics
• Risk assessment
• Genetic counseling
• Prenatal diagnosis and screening
• Personalized health care
• USMLE road map Genetics
• Mitochondrial dysfunction
• Congenital changes
• Genetics and cancer
• Genetics and common diseases
• Elseviers integrated review genetics
• Integrated review genetics
• Musculoskeletal disorders
• Neurologic diseases
• Cardiopulmonary disorders
• Modern molecular medicine
• Modern genetic techniques
• Molecular interactions
• Modular construction
• Extensions of mendelism
• The chromosomal basis of mendelism
• Variation in chromosome number
• The genetics of bacteria
• Summary of Doctoral Thesis
• Aquatic Products Processing
• Molecular genetics
• Shrimp breeding by growth trait
• Genetic variation
• Basic mechanisms
• Chromosomes in the cell
• Mechanisms of inheritance
• Hematologic genetics
• punicus Puzzle
• hydrophila
• Polymorphism
• Genomic Era
• Chronic Myelogenous Leukemia
• Colorectal Cancer
• Neurodegenerative Diseases
• Teratogenesi
• Medical Genetics
• Diagnostic Techniques
• Basic Genetics
• Mendels principles
• Mendels principles of heredity
• Mendels laws
• Single gene inheritance
• Chromosome theory
• Carriers of genes
• Gene linkage
• Mapping of genes
• DNA structure
• DNA replication
• DNA recombination
• Dissection through mutation
• Genetic analysis
• Gene structure
• Gene expression
• Protein synthesis
• Genetic code
• Digital analysis of DNA
• DNA fragmentation
• Restriction enzymes