tailieunhanh - Báo cáo khoa học: The N-linked oligosaccharides of aminopeptidase N from Manduca sexta

Mass spectrometric studies on the N-linked glycans of aminopeptidase 1 from Manduca sextahave revealed unusual structures not previously observed on any insect glycoprotein. Structure elucidation of these oligosaccha-rides was carried out by high-energy collision-induced dissociation (CID) using a matrix-assisted laser desorp-tion/ionization time-of-flight/time-of-flight (MALDI-TOF/ TOF) tandem mass spectrometer. These key experiments revealed that three out of the four N-linked glycosylation sites in this protein (Asn295, Asn623 and Asn752) are occupied with highly fucosylated N-glycans that possess unusual difucosylated cores. . | Eur. J. Biochem. 271 4241-4258 2004 FEBS 2004 doi The N-linked oligosaccharides of aminopeptidase N from Manduca sexta Site localization and identification of novel N-glycan structures Elaine Stephens1 Jane Suaars2 Sarah L Maslen1. Dudley H. Williams1 Len C Packman2 and David J Ellar2 B 4 BF B F BF B B BF B B F B B B F BB wF F S F BB B BB B B . B B B BB B B B w BB B V BB. WHBBBB BB B B B B B BB . B BB B Bl B B BB B B BB B B BB B F BB w B BB IB . B B BB B 1 Department of Chemistry and department of Biochemistry University of Cambridge UK Mass spectrometric studies on the N-linked glycans of aminopeptidase 1 from Manduca sexta have revealed unusual structures not previously observed on any insect glycoprotein. Structure elucidation of these oligosaccharides was carried out by high-energy collision-induced dissociation CID using a matrix-assisted laser desorp-tion ionization time-of-flight time-of-flight MALDI-TOF TOF tandem mass spectrometer. These key experiments revealed that three out of the four N-linked glycosylation sites in this protein Asn295 Asn623 and Asn752 are occupied with highly fucosylated N-glycans that possess unusual difucosylated cores. Cross-ring fragment ions and internal fragment ions observed in the CID spectra showed that these fucoses are found at the 3-position of proximal GlcNAc and at the 3-position of distal GlcNAc in the chitobiose unit. The latter substitution has only been previously observed in nematodes. In addition these core structures can be decorated with novel fucosylated antennae composed of Fuca 1-3 GlcNAc. Key fragment ions revealed that these antennae are predominantly found on the upper 6-arm of the core mannose. The paucimannosidic N-glycan Man3GlcNAc2 commonly found on other insect glycoproteins is the predominant oligosaccharide found at the remaining N-glycosylation site Asn609 . Keywords aminopeptidase N fucose high-energy CID insect glycosylation MALDI-TOF TOF. As in other eukaryotic .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• medicine
• cell proliferation
• oxidation
• Selective Oxidation
• Gold Nanoparticles
• Aerobic Oxidation
• Oxidation Catalysts
• Crystalline Materials
• Molecular Oxygen
• Nuclear engineering and technology
• The recycling of nuclear graphite after micro oxidation
• Nuclear graphite after micro oxidation
• Whether ground micro oxidation graphite
• The oxidized graphite particles
• The recycling of nuclear graphite
• Dietary fructose
• Fatty acid oxidation
• Hepatic steatosis
• Fatty acid β oxidation
• Lipid oxidation
• Meal extracts
• Refined soybean oil
• Total oxidation value
• Meal extracts on retarding lipid oxidation
• Environmental advances
• Volatile organic compounds
• Ozone oxidation technique
• Catalytic ozone oxidation
• Ozone oxidation
• breast cancer
• disorders
• Fatty acid
• Vietnam Journal of Science and Technology
• Masterbatch containing anti oxidation additive
• Anti oxidation master batch
• Melt flow index
• Tensile strength and elongation
• Vietnam Journal of Chemistry
• Mg Al MoO4 layered double hydroxides
• Catalysts for the oxidation of styrene
• The oxidation of styrene
• The synthesized solids showed the layered hydroxide structure
• Effects of ascorbic acid treatment
• Packaging method on lipid oxidation
• Frozen storage
• Ascorbic acid
• Vacuum packaging
• Lipid oxidation
• CO oxidation
• Manganese oxides
• Binary catalysts
• Formic acid oxidation
• Novel binary catalysts
• Thermal spray
• NiCr Al duplex coating
• Oxidation resistance
• Carbon steel substrate
• High temperature oxidation resistant
• High temperature working parts
• High temperature steam oxidation kinetics
• High temperature oxidation
• Weight gain measurements
• E110 cladding tubes material
• Copper oxide
• Thin films
• Thermal oxidation
• Annealing temperature
• Thermal oxidation method
• X ray diffraction measurement
• Journal of Chemistry
• Aftertreatment systems
• Diesel oxidation catalyst
• Bimetallic catalyst
• Commercial monolith
• Journal of Science and Technology
• Plasma electrolytic oxidation coating
• 6061 Al alloy
• Electrolyte without alkali ions
• Plasma electrolytic oxidation
• Porous nickel
• Cast nickel
• Duplex macrostructure
• Inward migration
• Linear kinetics
• Redox process
• Oxidation reduction reactions
• Oxidation reduction reactions in geochemistry
• Extremely reducing or oxidizing conditions
• Reduction potentials in geochemistry
• Benzyl alcohol oxidation
• Peripherally 1 2 dinaphthyl ethanediol
• Phthalocyanine complexes
• UV Vis light absorption
• Catalytic oxidation
• polyamine
• oxidase K300M
• Crystal structure
• bacteria
• scientific research
• biochemistry
• hemoglobin
• biopreservation
• cytoplasm
• chemical reaction
• tumor cells