tailieunhanh - Báo cáo khoa học: Investigation of the contribution of histidine 119 to the conduction of protons through human Nox2

The conduction of protons through human Nox2 has pre-viously been shown to be dependent upon His115. Align-ment of sequences for both animal and plant Nox proteins indicated that histidines 115 and 119 are both highly con-served, while His111 was conserved among animal homo-logues of Nox1–4. To investigate the possible role that these histidine residues might play in the conduction of protons through Nox2, we have introduced both paired and single mutations into these histidine residues. Each construct was used to generate a CHO cell line in which the expression of themutatedNox2was assessed | Eur. J. Biochem. 271 4026-4033 2004 FEBS 2004 doi Investigation of the contribution of histidine 119 to the conduction of protons through human Nox2 Tosti J. Mankelow X. Wen Hu Kate Adamsf and Lydia M. Henderson Department of Biochemistry School of Medical Sciences University of Bristol UK The conduction of protons through human Nox2 has previously been shown to be dependent upon His115. Alignment of sequences for both animal and plant Nox proteins indicated that histidines 115 and 119 are both highly conserved while His111 was conserved among animal homologues of Nox1-4. To investigate the possible role that these histidine residues might play in the conduction of protons through Nox2 we have introduced both paired and single mutations into these histidine residues. Each construct was used to generate a CHO cell line in which the expression of the mutated Nox2 was assessed. Nox2 was expressed in each of the CHO cell lines generated however the level of expression of H111 115L in CHO cells was lower and that of H111L very much reduced compared to that of wild-type Nox2. The arachidonic acid activated proton flux was absent in the CHO cell lines expressing the mutations of H111 115L H111 119L or H115 119L compared to that observed for wild-type Nox2. Similarly only a small efflux of protons was observed from CHO cells expressing either H119L or H111L. In all cases the expected proton flux was elicited through the addition of the protonophore carbonyl cyanide m-chlorophenylhydrazone. Conclusions regarding the role of His111 in the conduction of protons cannot be drawn due to the reduced expression. We can however conclude that His119 in addition to His115 is required for the conduction of protons through Nox2. His119 has been identified as a highly conserved residue for which no function has previously been proposed. Keywords gp91phox membranes NADPH oxidase proton transport. The phagocytic white blood cells engulf kill and digest .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• intermediate cells
• cell proliferation
• medical analysis
• thesis report
• medical report
• medical knowledge
• medical research
• analysis of cytoplasmic
• environmental medicine
• raccoon experiments
• Content and Language Integrated Learning
• Analysis of term extraction for English
• English medical texts
• Vietnamese medical experts
• Educational philosophy across Europe
• Medical image analysis
• Computer aided diagnosis
• FLAIR magnetic resonance imaging
• Realistic lesion insertion
• Medical data augmentation
• Diffuse lung diseases
• Tomosynthesis imaging
• Spinal abnormalities
• Health informatics
• Medical microwave imaging
• statements and documents scientific reports
• medical reports
• clinical trials
• medical management
• health care
• scientific research
• health policy
• pathology
• anatomy
• health education