tailieunhanh - Báo cáo khoa học: Solution structure of IsTX A male scorpion toxin fromOpisthacanthus madagascariensis(Ischnuridae)

The novel sex-specific potassium channel inhibitor IsTX, a 41-residue peptide, was isolated from the venom of male Opisthacanthus techniques revealed that the structure of IsTX contains a cysteine-stabilizeda/b-fold. IsTX is classified, based on its sequential and structural similarity, in the scorpion short toxin familya-KTx6. Thea-KTx6 family contains a single a-helix and twob-strands connected by four disulfide brid-ges and binds to voltage-gated K + channels and apamin-sensitive Ca 2+ -activated K + channels. . | Eur. J. Biochem. 271 3855-3864 2004 FEBS 2004 doi Solution structure of IsTX A male scorpion toxin from Opisthacanthus madagascariensis Ischnuridae Nahoko Yamaii1. Li Dai1 Kenji Suaase1 Marta Andriantsiferana2 Terumi Nakaiima1 and Takashi Iwashita1 1Suntory Institute for Bioorganic Research Mishima-Gun Osaka Japan 2Faculty of Science University of Antananarivo Madagascar The novel sex-specific potassium channel inhibitor IsTX a 41-residue peptide was isolated from the venom of male Opisthacanthus madagascariensis. Two-dimensional NMR techniques revealed that the structure of IsTX contains a cysteine-stabilized a b-fold. IsTX is classified based on its sequential and structural similarity in the scorpion short toxin family a-KTx6. The a-KTx6 family contains a single a-helix and two b-strands connected by four disulfide bridges and binds to voltage-gated K channels and apamin-sensitive Ca2 -activated K channels. The three-dimensional structure of IsTX is similar to that of Heterometrus spinifer toxin HsTX1 . HsTX1 blocks the channel at picomolar concentrations whereas IsTX has much lower affinities 10 000-fold . To investigate the structure-activity relationship the geometry of sidechains and electrostatic surface potential maps were compared with HsTX1. As a result of the comparison of the primary structures Lys27 of IsTX was conserved at the same position in HsTX1. The analogous Lys23 of HsTX1 the most critical residue for binding to potassium channels binds to the channel pore. However IsTX has fewer basic residues to interact with acidic channel surfaces than HsTX1. MALDI-TOF MS analysis clearly indicated that IsTX was found in male scorpion venom but not in female. This is the first report that scorpion venom contains sex-specific compounds. Keywords cysteine-stabilized a b-fold NMR potassium channels scorpion toxin sex-specific toxin. The venom of Opisthacanthus nutdagascariensis scorpions from the Ischnuridae family .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• molecular Biology
• tumor cells
• Antimicrobial
• Nelson’s pediatric antimicrobial therapy
• Choosing among antifungal agents
• Antimicrobial therapy for newborns
• Antimicrobial therapy according
• Clinical syndromes
• Beta lactamase inhibitors
• Antimicrobial therapy
• Mycobacterial pathogens
• Specific bacterial
• Nosocomial infection
• Hospital acquired infection
• Prevalence and antimicrobial susceptibility pattern
• Antimicrobial susceptibility pattern
• Evaluate antimicrobial susceptibility pattern
• Antimicrobial properties
• Antimicrobial activity
• Phyo chemical analysis
• Plant resources having antimicrobial properties obtained
• Phytochemical analysis of plant resources
• Antimicrobial activity of ZITRITIDE
• Organic antimicrobial fogging solution
• Special reference
• Infection prevention
• Hospital environments
• Other clean room facilities
• Specific parasitic pathogens
• Antimicrobial susceptibility
• Changing antimicrobial susceptibility pattern
• Pus samples
• Susceptibility pattern
• Antimicrobial susceptibility testing
• BMC Veterinary Research
• Antimicrobial usage
• Antimicrobial resistance
• Salmonella spp
• Alphabetic listing of antimicrobials
• Adverse reactions to antimicrobial agents
• Drug interactions
• Antibiotic therapy
• Alphabetic listing
• Symptomatic infection
• Antimicrobial prophylaxis
• Adverse reactions
• Lecture Microbiology
• Roles of microbes
• Biological research
• Antimicrobial drug properties
• Drug target sites
• Journal of Chemistry
• The Chemistry and Antimicrobial Activity of Psychotria reevesii Wall
• The Chemistry and Antimicrobial Activity
• Psychotria reevesii Wall
• The polar fractions against Staphylococcus aureus
• Journal of medical research
• Prevalence of antimicrobial resistance of neisseria gonorrhoeae
• Antimicrobial resistance of neisseria gonorrhoeae
• National hospital of dermatology and venereology in 2017
• Minimum inhibitory concentration
• Antimicrobial compound
• Burkholderia paludis
• Enterococcus faecalis
• The role of reactive oxygen species
• Pentafluorophenylammonium triflate
• Coumarin 3 carboxylic acid
• Chromeno[2 3 d]pyrimidinones
• 4H pyran 5 ethylcarboxylate
• Green synthesis
• Characterization and antimicrobial evaluation
• Antimicrobial evaluation
• Nano silver
• Mastitis causing microorganisms
• Bacterial profile
• Ventilator associated pneumonia
• BMC Pediatrics
• Pediatric community acquired pneumonia
• Community acquired pneumonia
• Empiric antimicrobial choice
• Heightened antimicrobial stewardship
• Virulence genes
• Identification and antimicrobial resistance
• Enterococcus faecalis isolated
• Diabetic foot patients
• ESKAPE pathogens
• Multidrug resistant (MDR) bacteria
• Tertiary care centre
• Antimicrobial susceptibility profile
• Clinical importance
• Staphylococcus aureus
• Hadman farm dairies
• Antimicrobial susceptibility test
• Cheese from Hadman farm dairies
• BMC Pharmacology and Toxicology
• MDR Salmonella
• Antimicrobial resistant Salmonella
• Pan resistance
• Drug sensitivity
• Amhara National Regional State
• Clinical microbiology laboratories
• Risk factors
• Mount Sinai Beth Israel
• Outpatient parenteral antimicrobial therapy
• Bacillus firmicutes
• Biopeptide or Bacterio cin
• Food borne pathogens