tailieunhanh - Báo cáo khoa học: Irregular dimerization of guanylate cyclase-activating protein 1 mutants causes loss of target activation

Guanylate cyclase-activating proteins (GCAPs) are neur-onal calcium sensors that activate membrane bound guanylate cyclases (EC .) of vertebrate photoreceptor cells when cytoplasmic Ca 2+ decreases during illumination. GCAPs contain fourEF-handCa 2+ -bindingmotifs, but the first EF-hand is nonfunctional. It was concluded that for GCAP-2, the loss of Ca 2+ -binding ability of EF-hand 1 resulted in a region that is crucial for targeting guanylate cyclase [Ermilov,, . (2001) J. Biol. , 48143–48148]. In this study we tested the consequences of mutations in EF-hand 1 of GCAP-1 with respect to Ca 2+ binding, Ca 2+ -induced con-formational changes and target activation. . | Eur. J. Biochem. 271 3785-3793 2004 FEBS 2004 doi Irregular dimerization of guanylate cyclase-activating protein 1 mutants causes loss of target activation Ji-Young Hwang1 Ramona Schlesinger2 and Karl-Wilhelm Koch1 1Institut fur Biologische Informationsverarbeitung 1 and 22 Forschungszentrum Jiilich Germany Guanylate cyclase-activating proteins GCAPs are neuronal calcium sensors that activate membrane bound guanylate cyclases EC . of vertebrate photoreceptor cells when cytoplasmic Ca2 decreases during illumination. GCAPs contain four EF-hand Ca2 -binding motifs but the first EF-hand is nonfunctional. It was concluded that for GCAP-2 the loss of Ca2 -binding ability of EF-hand 1 resulted in a region that is crucial for targeting guanylate cyclase Ermilov . Olshevskaya . Dizhoor . 2001 J. Biol. Chem. 276 48143-48148 . In this study we tested the consequences of mutations in EF-hand 1 of GCAP-1 with respect to Ca2 binding Ca2 -induced conformational changes and target activation. When the nonfunctional first EF-hand in GCAP-1 is replaced by a functional EF-hand the chimeric mutant CaM-GCAP-1 bound four Ca2 and showed similar Ca2 -dependent changes in tryptophan fluorescence as the wild-type. CaM-GCAP-1 neither activated nor interacted with guanylate cyclase. Size exclusion chromatography revealed that the mutant tended to form inactive dimers instead of active monomers like the wild-type. Critical amino acids in EF-hand 1 of GCAP-1 are cysteine at position 29 and proline at position 30 as changing these to glycine was sufficient to cause loss of target activation without a loss of Ca2 -induced conformational changes. The latter mutation also promoted dimerization of the protein. Our results show that EF-hand 1 in wild-type GCAP-1 is critical for providing the correct conformation for target activation. Keywords GCAP guanylate cyclase neuronal Ca2 sensor phototransduction. Light triggers the activation of a G-protein .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• immune system
• Antimicrobial
• biosynthesis
• Olea europaea
• Vitamin E biosynthesis
• Gene expression
• Tocopherol biosynthesis genes
• Synthesized in plants
• Mango malformation
• Transcriptomic analysis of Brassinosteroid biosynthesis
• Brassinosteroid biosynthesis
• Signaling pathway genes
• Malformed tissues
• Mango variety amrapalli
• Tartary buckwheat
• Jasmonate ZIM domain proteins
• Yeast two hybrid
• Anthocyanin biosynthesis
• Flavonoid biosynthesis
• Plant Biology
• Camellia sinensis
• Integrated transcriptomics
• Metabolomics analysis
• Theanine biosynthesis
• biochemistry
• molecular Biology
• Trypanosoma
• medical research
• breast cancer
• medicine
• Structural Biology
• intermediate cells
• metabolites
• biochemical studies
• medical reports
• health education
• Glutathionylspermidine is an intermediate formed in the biosynthesis of trypanothione
• an essential metabolite in defence against chemical and oxi dative stress in the Kinetoplastida
• The kinetic mechanism for glutathionyl spermidine synthetase (EC 6
• 8) from Crithidia fasciculata (CfGspS) obeys a rapid equilibrium random ter ter model with kinetic constants KGSH= 609lm
• KSpd= 157lmand KATP= 215lm
• scientific research
• oxidation
• cytoplasm
• biological cells
• Streptomyces
• molecular
• analysis of cytoplasmic
• hemoglobin
• Ebook Biochemistry
• Synthesizing themolecules of life
• The biosynthesis of amino acids
• The biosynthesis of membrane lipids
• Responding to environmental changes
• Sensory systems
• Tạp chí Đại học công nghiệp thực phẩm
• Effect of plant oils on mycelial biomass production
• Biosynthesis and antioxidants of exopolysaccharide
• Immunomodulatory and antitumor
• Demonstrated that exopolysaccharides
• Vietnam Journal of Science and Technology
• Rice bran oil on mycelial biomass production
• Biosynthesis and bioactivities of polysaccharides
• Ophiocordyceps sinensis fungus
• Rice bran oil
• Daucus carota
• MYB 6 and Restriction digestion
• Regulating Anthocyanin biosynthesis
• GFP Tagged MYB 6 Gene
• Aimed to clone GFP
• BMC Plant Biology
• P appendiculatum
• Flavonoids biosynthesis
• Basic helix loop helix
• Transcription factors
• Pyrus bretschneideri
• Stone cells
• Lignin biosynthesis
• Transcriptomic analysis
• Cinnamoyl CoA reductase
• Solanum melongena L
• High temperature
• BMC Biotechnology
• Tribolium castaneum
• RNA interference
• Chitin biosynthesis pathway
• Gene function
• Gossypol biosynthesis
• Plant grafting
• Organ culture
• Gene expression profiling
• RNA seq
• Ester biosynthesis
• Transcriptional regulation
• Ripening fruit
• Amaryllidaceae alkaloid
• Pathogenesis related protein 10
• Alkaloid biosynthesis
• Narcissus pseudonarcissus
• Norcoclaurine synthase
• Inflorescence architecture
• BRs biosynthesis
• Panicle architecture
• PMM1 transcripts
• Tissue acidification
• Litchi chinensis
• Rubber biosynthesis
• Parthenium argentatum
• Hevea plantations