tailieunhanh - Báo cáo khoa học: Fatty acids increase the circulating levels of oxidative stress factors in mice with diet-induced obesity via redox changes of albumin

Plasma concentrations of free fatty acids are increased in metabolic syn-drome, and the increased fatty acids may cause cellular damage via the induction of oxidative stress. The present study was designed to determine whether the increase in fatty acids can modify the free sulfhydryl group in position 34 of albumin (Cys34) and enhance the redox-cycling activity of the copper–albumin complex in high-fat diet-induced obese mice. | ỊFEBS Journal Fatty acids increase the circulating levels of oxidative stress factors in mice with diet-induced obesity via redox changes of albumin Mayumi Yamato1 Takeshi Shiba1 Masayoshi Yoshida2 Tomomi Ide2 Naoko Seri1 Wataru Kudou1 Shintaro Kinugawa3 and Hiroyuki Tsutsui3 1 Department REDOX MedicinalScience Faculty of PharmaceuticalSciences Kyushu University Fukuoka Japan 2 Department of Cardiovascular Medicine Graduate Schoolof MedicalSciences Kyushu University Fukuoka Japan 3 Department of Cardiovascular Medicine Hokkaido University Graduate Schoolof Medicine Sapporo Japan Keywords ESR albumin fatty acid obesity oxidative stress Correspondence H. Tsutsui Department of Cardiovascular Medicine Hokkaido University Graduate Schoolof Medicine Kita-15 Nishi-7 Kita-ku Sapporo 060-8638 Japan Fax 81 11 706 7874 Tel 81 11 706 6973 E-mail htsutsui@ Received 11 April2007 revised 29 May 2007 accepted 31 May 2007 doi Plasma concentrations of free fatty acids are increased in metabolic syndrome and the increased fatty acids may cause cellular damage via the induction of oxidative stress. The present study was designed to determine whether the increase in fatty acids can modify the free sulfhydryl group in position 34 of albumin Cys34 and enhance the redox-cycling activity of the copper-albumin complex in high-fat diet-induced obese mice. The mice were fed with commercial normal diet or high-fat diet and water ad libitum for 3 months. The high-fat diet-fed mice developed obesity hyperlipemia and hyperglycemia. The plasma fatty acid albumin ratio also significantly increased in high-fat diet-fed mice. The increased fatty acid albumin ratio was associated with conformational changes in albumin and the oxidation of sulfhydryl groups. Moreover an ascorbic acid radical an index of redox-cycling activity of the copper-albumin complex was detected only in the plasma from obese mice whereas the plasma concentrations of ascorbic acid