tailieunhanh - Báo cáo khoa học: Characterization and expression analysis of the aspartic protease gene family of Cynara cardunculus L.

Cardosin A and cardosin B are two aspartic proteases mainly found in the pistils of cardoonCynara cardunculusL., whose flowers are traditionally used in several Mediterranean countries in the manufacture of ewe’s cheese. We have been characterizing cardosins at the biochemical, structural and molecular levels. | ỊFEBS Journal Characterization and expression analysis of the aspartic protease gene family of Cynara cardunculus L. Catarina Pimentel1 2 3 Dominique Van Der Straeten3 Euclides Pires1 4 Carlos Faro1 4 and Claudina Rodrigues-Pousada2 1 Departamento de Biologia Molecular e Biotecnologia do Centro de Neurociencias de Coimbra Universidade de Coimbra Portugal 2 Instituto de Tecnologia Quimica e Biologica Universidade Nova de Lisboa Oeiras Portugal 3 Unit Plant Hormone Signalling and Bio-imaging Ghent University Belgium 4 Departamento de Bioquimica Faculdade de Ciencias e Tecnologia Universidade de Coimbra Portugal Keywords aspartic proteases cardosin leader intron pistil promoter Correspondence C. Rodrigues-Pousada Instituto de Tecnologia Quimica e Biologica Apt. 127 2781-901 Oeiras Portugal Fax 351 214433644 Tel 351 214469624 E-mail claudina@ C. Faro Departamento de Bioquimica Faculdade de Ciencias e Tecnologia Universidade de Coimbra Apt. 3126 3000 Coimbra Portugal Fax 351 230480208 Tel 351 239480210 E-mail cfaro@ Database The nucleotide sequences of Cynara cardun-culus L. aspartic protease genes have been submitted to the EBI Data Bank under the accession numbers AM286227 cardosin B and AM286279 cyprosin B Cardosin A and cardosin B are two aspartic proteases mainly found in the pistils of cardoon Cynara cardunculus L. whose flowers are traditionally used in several Mediterranean countries in the manufacture of ewe s cheese. We have been characterizing cardosins at the biochemical structural and molecular levels. In this study we show that the cardoon aspartic proteases are encoded by a multigene family. The genes for cardosin A and cardo-sin B as well as those for two new cardoon aspartic proteases designated cardosin C and cardosin D were characterized and their expression in C. cardunculus L. was analyzed by RT-PCR. Together with cardosins a partial clone of the cyprosin B gene was isolated revealing that cardosin and cyprosin genes .

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