tailieunhanh - Báo cáo khoa học: Comparison of membrane fraction proteomic profiles of normal and cancerous human colorectal tissues with gel-assisted digestion and iTRAQ labeling mass spectrometry

The aim of this study was to uncover the membrane protein profile differ-ences between colorectal carcinoma and neighboring normal mucosa from colorectal cancer patients. Information from cellular membrane proteomes can be used not only to study the roles of membrane proteins in fundamen-tal biological processes, but also to discover novel targets for improving the management of colorectal cancer patients. | Comparison of membrane fraction proteomic profiles of normal and cancerous human colorectal tissues with gel-assisted digestion and iTRAQ labeling mass spectrometry Jinn-Shiun Chen1 2 Kuei-Tien Chen3 Chung-Wei Fan2 4 Chia-Li Han5 Yu-Ju Chen5 Jau-Song Yu6 Yu-Sun Chang7 Chih-Wei Chien5 Chien-Peng Wu5 Ray-Ping Hung3 and Err-Cheng Chan3 1 Department of Surgery Chang Gung Memorial Hospital Tao Yuan Taiwan 2 College of Medicine Chang Gung University Tao Yuan Taiwan 3 Department of MedicalBiotechnology and Laboratory Science Chang Gung University Tao Yuan Taiwan 4 Department of ColorectalSurgery Chang Gung Memorial Hospital Keelung Taiwan 5 Institute of Chemistry Academia Sinica Taipei Taiwan 6 Department of Celland Molecular Biology Chang Gung University Tao Yuan Taiwan 7 Molecular Medicine Research Center Chang Gung University Tao Yuan Taiwan Keywords biomarker colorectal cancer mass spectrometry membrane protein proteomic profile Correspondence . Chan Department of Medical Biotechnology and Laboratory Science Chang Gung University 259 Wen-Hua 1st Road Kweishan Taoyuan Taiwan China Fax 886 3 2118741 Tel 886 3 2118800 ext. 5220 E-mail chanec@ Note Jinn-Shiun Chen Kuei-Tien Chen and Chung-Wei Fan contributed equally to this article Received 12 January 2010 revised 9 April 2010 accepted 17 May 2010 doi The aim of this study was to uncover the membrane protein profile differences between colorectal carcinoma and neighboring normal mucosa from colorectal cancer patients. Information from cellular membrane proteomes can be used not only to study the roles of membrane proteins in fundamental biological processes but also to discover novel targets for improving the management of colorectal cancer patients. We used solvent extraction and a gel-assisted digestion method together with isobaric tags with related and absolute quantitation iTRAQ reagents to label tumoral and adjacent normal tissues in a pairwise manner n 8 . For .