tailieunhanh - Báo cáo khoa học: The two Caenorhabditis elegans metallothioneins (CeMT-1 and CeMT-2) discriminate between essential zinc and toxic cadmium

The nematodeCaenorhabditis elegansexpresses two metallothioneins (MTs), CeMT-1 and CeMT-2, that are believed to be key players in the protection against metal toxicity. In this study, both isoforms were expressedin vitro in the presence of either Zn(II) or Cd(II). | ỊFEBS Journal The two Caenorhabditis elegans metallothioneins CeMT-1 and CeMT-2 discriminate between essential zinc and toxic cadmium Sukaina Zeitoun-Ghandour1 John M. Charnock2 Mark E. Hodson3 Oksana I. Leszczyszyn4 Claudia A. Blindauer4 and Stephen R. Sturzenbaum1 1 Schoolof Biomedical Health Sciences King s College London UK 2 Schoolof Earth Atmospheric and EnvironmentalSciences University of Manchester UK 3 Department of SoilScience University of Reading UK 4 Department of Chemistry University of Warwick Coventry UK Keywords affinity C. elegans cadmium metal speciation metallothionein zinc Correspondence S. Sturzenbaum Schoolof Biomedical Health Sciences PharmaceuticalScience Division King s College London 150 Stamford Street London SE1 9NH UK Fax 44 2078484500 Tel. 44 2078484406 E-mail Received 22 January 2010 revised 23 March 2010 accepted 30 March 2010 doi The nematode Caenorhabditis elegans expresses two metallothioneins MTs CeMT-1 and CeMT-2 that are believed to be key players in the protection against metal toxicity. In this study both isoforms were expressed in vitro in the presence of either Zn II or Cd II . Metal binding stoichiometries and affinities were determined by ESI-MS and NMR respectively. Both isoforms had equal zinc binding ability but differed in their cadmium binding behaviour with higher affinity found for CeMT-2. In addition wild-type C. elegans single MT knockouts and a double MT knockout allele were exposed to zinc 340 pM or cadmium 25 pM to investigate effects in vivo. Zinc levels were significantly increased in all knockout strains but were most pronounced in the CeMT-1 knockout mtl-1 tm1770 while cadmium accumulation was highest in the CeMT-2 knockout mtl-2 gk125 and the double knockout mtl-1 mtl-2 zs1 . In addition metal speciation was assessed by X-ray absorption fine-structure spectroscopy. This showed that O-donating probably phosphate-rich ligands play a dominant .

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