tailieunhanh - Báo cáo khoa học: Structural basis for the interaction between dynein light chain 1 and the glutamate channel homolog GRINL1A

Human dynein light chain 1 (DYNLL1) is a dimeric 89-residue protein that is known to be involved in cargo binding within the dynein multiprotein complex. Over 20 protein targets, of both cellular and viral origin, have been shown to interact with DYNLL1, and some of them are transported in a retrograde manner along microtubules. | ễFEBS Journal Structural basis for the interaction between dynein light chain 1 and the glutamate channel homolog GRINL1A Maria F. Garcia-Mayoral1 Monica Martinez-Moreno2 Juan P. Albar3 Ignacio Rodriguez-Crespo2 and Marta Bruix1 1 Departamento de Espectroscopia y Estructura Molecular Institute de Quimica-Fisica Rocasolano Consejo Superior de Investigaciones Cientificas CSIC Madrid Spain 2 Departamento de Bioquimica y Biologia Molecular I Universidad Complutense Madrid Spain 3 Proteomics Facility Centro Nacionalde Biotecnologia Consejo Superior de Investigaciones Cientificas Madrid Spain Keywords dynein light chain glutamate channel homolog NMR protein-protein interactions Correspondence I. Rodriguez-Crespo Departamento de Bioquimica y Biologia Molecular I Universidad Complutense 28040 Madrid Spain Fax 34 913944159 Tel 34 913944137 E-mail nacho@ M. Bruix Departamento de Espectroscopia y Estructura Molecular Instituto de Quimica-Fisica Rocasolano CSIC Serrano 119 28006 Madrid Spain Fax 34 915642431 Tel 34 917459511 E-mail mbruix@ Received 12 January 2010 revised 11 March 2010 accepted 15 March 2010 doi Human dynein light chain 1 DYNLL1 is a dimeric 89-residue protein that is known to be involved in cargo binding within the dynein multiprotein complex. Over 20 protein targets of both cellular and viral origin have been shown to interact with DYNLL1 and some of them are transported in a retrograde manner along microtubules. Using DYNLL1 as bait in a yeast two-hybrid screen with a human heart library we identified GRINL1A ionotropic glutamate receptor N-methyl-D-aspartate-like 1A a homolog of the ionotropic glutamate receptor N-methyl D-aspartate as a DYNLL1 binding partner. Binding of DYNLL1 to GRINL1A was also demonstrated using GST fusion proteins and pepscan membranes. Progressive deletions allowed us to narrow the DYNLL1 binding region of GRINL1A to the sequence REIGVGCDL. Combining these results with NMR data