tailieunhanh - Báo cáo khoa học: The ROQUIN family of proteins localizes to stress granules via the ROQ domain and binds target mRNAs

Roquin is an E3 ubiquitin ligase with a poorly understood but essential role in preventing T-cell-mediated autoimmune disease and in microRNA-mediated repression of inducible costimulator (Icos) mRNA. Roquin and its mammalian paralogue membrane-associated nucleic acid binding protein (MNAB) define a protein family distinguished by an 200 amino acid domain of unknown function, ROQ, that is highly conserved from mam-mals to invertebrates and is flanked by a RING-1 zinc finger and a CCCH zinc finger | ỊFEBS Journal The ROQUIN family of proteins localizes to stress granules via the ROQ domain and binds target mRNAs Vicki Athanasopoulos1 2 Andrew Barker3 Di Yu4 Andy H-M. Tan1 Monika Srivastava1 Nelida Contreras2 Jianbin Wang2 Kong-Peng Lam5 Simon H. J. Brown6 Christopher C. Goodnow1 Nicholas E. Dixon6 Peter J. Leedman3 7 Robert Saint2 8 and Carola G. Vinuesa1 1 The John Curtin Schoolof MedicalResearch The Australian National university Canberra Australia 2 ARC SpecialResearch Centre for the Molecular Genetics of Development CMGD Research Schoolof Biology Canberra Australia 3 Laboratory for Cancer Medicine The University of Western Australia Centre for MedicalResearch Western Australian Institute for Medical Research Perth Australia 4 Immunology and Inflammation Research Program Garvan Institute of MedicalResearch Sydney Australia 5 Laboratory of Immunology Bioprocessing Technology Institute Agency for Science Technology and Research A STAR Singapore 6 Schoolof Chemistry University of Wollongong Australia 7 Schoolof Medicine and Pharmacology The University of Western Australia Crawley Australia 8 Department of Genetics University of Melbourne Australia Keywords membrane-associated nucleic acid binding protein microRNA ROQ ROQUIN stress granules Correspondence . Vinuesa The John Curtin Schoolof MedicalResearch The Australian National University Garran Road PO Box 34 ACT 2601 Australia Fax 61 2 6125 2595 Tel 61 2 6125 4500 E-mail These authors contributed equally to this article Received 22 December 2009 revised 18 February 2010 accepted 25 February 2010 doi Roquin is an E3 ubiquitin ligase with a poorly understood but essential role in preventing T-cell-mediated autoimmune disease and in microRNA-mediated repression of inducible costimulator Icos mRNA. Roquin and its mammalian paralogue membrane-associated nucleic acid binding protein MNAB define a protein family distinguished by an 200 amino acid domain