tailieunhanh - Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information

Antibodies and their fragments are attractive binding proteins because their high binding strength is generated by several hypervariable loop regions, and because high-quality libraries can be prepared from the vast gene clus-ters expressed by mammalian lymphocytes. Recent explorations of new genome sequences and protein structures have revealed various small, nonantibody scaffold proteins. | MINIREVIEW Protein-protein interactions and selection generation of molecule-binding proteins on the basis of tertiary structural information HU . II . 12 -I- I I K I I I 3 A 1 1 I II. 1 II IX 1 Mitsuo Umetsu Takeshi Nakanishi Ryutaro Asano Takamitsu Hattori and Izumi Kumagai 1 Department of Biomolecular Engineering Graduate Schoolof Engineering Tohoku University Sendai Japan 2 Center for Interdisciplinary Research Tohoku University Sendai Japan 3 Department of Applied Chemistry and Bioengineering Graduate Schoolof Engineering Osaka City University Japan Keywords library design peptide grafting protein structure scaffold protein Correspondence M. Umetsu Department of Biomolecular Engineering Graduate School of Engineering Tohoku University Aoba 6-6-11 Aramaki Aoba-ku Sendai 980-8579 Japan Fax 81 22 795 7276 Tel 81 22 795 7276 E-mail mitsuo@ Received 29 October 2009 revised 5 February 2010 accepted 24 February 2010 doi Antibodies and their fragments are attractive binding proteins because their high binding strength is generated by several hypervariable loop regions and because high-quality libraries can be prepared from the vast gene clusters expressed by mammalian lymphocytes. Recent explorations of new genome sequences and protein structures have revealed various small nonantibody scaffold proteins. Accurate structural descriptions of proteinprotein interactions based on X-ray and NMR analyses allow us to generate binding proteins by using grafting and library techniques. Here we review approaches for generating binding proteins from small scaffold proteins on the basis of tertiary structural information. Identification of binding sites from visualized tertiary structures supports the transfer of function by peptide grafting. The local library approach is advantageous as a go-between technique for grafted foreign peptide sequences and small scaffold proteins. The identification of binding sites also supports .

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