tailieunhanh - Báo cáo khoa học: Ferrous Campylobacter jejuni truncated hemoglobin P displays an extremely high reactivity for cyanide – a comparative study

Campylobacter jejunihosts two hemoglobins (Hbs). TheCamplylobacter je-juni single-domain Hb (called Cgb) is homologous to the globin domain of flavohemoglobin, and it has been proposed to protect the bacterium against nitrosative stress. | ỊFEBS Journal Ferrous Campylobacter jejuni truncated hemoglobin P displays an extremely high reactivity for cyanide -a comparative study Alessandro Bolli1 Chiara Ciaccio2 3 Massimo Coletta2 3 Marco Nardini4 Martino Bolognesi4 Alessandra Pesce5 Michel Guertin6 Paolo Visca1 7 and Paolo Ascenzi1 7 1 Dipartimento di Biologia and Laboratorio Interdipartimentale di Microscopia Elettronica Universita Roma Tre Italy 2 Dipartimento di Medicina Sperimentale e Scienze Biochimiche University di Roma Tor Vergata Italy 3 Consorzio Interuniversitario di Ricerca in Chimica dei Metalli nei Sistemi Biologici Bari Italy 4 Dipartimento di Scienze Biomolecolari e Biotecnologie and CNR-INFM University di Milano Italy 5 Dipartimento di Fisica CNR-INFM and Centro di Eccellenza per la Ricerca Biomedica Universita di Genova Italy 6 Departement de Biochimie et de Microbiologie Universite Laval Faculte des Sciences et de Genie Quebec Canada 7 Istituto Nazionale per le Malattie Infettive IRCCS Lazzaro Spallanzani Roma Italy Keywords Campylobacter jejuni truncated hemoglobin P cyanide binding kinetics Mycobacterium tuberculosis truncated hemoglobin N and O thermodynamics Correspondence P. Ascenzi Dipartimento di Biologia Universita Roma Tre Viale G. Marconi 446 I-00146 Roma Italy Fax 39 06 5517 6321 Tel 39 06 5517 3200 2 E-mail ascenzi@ Received 7 September 2007 revised 28 November 2007 accepted 7 December 2007 doi Campylobacter jejuni hosts two hemoglobins Hbs . The Camplylobacter jejuni single-domain Hb called Cgb is homologous to the globin domain of flavohemoglobin and it has been proposed to protect the bacterium against nitrosative stress. The second Hb is called Ctb hereafter Cj-trHbP belongs to truncated Hb group III and has been hypothesized to be involved in O2 chemistry. Here the kinetics and thermodynamics of cyanide binding to ferric and ferrous Cj-trHbP Cj-trHbP III and Cj-trHbP II respectively are reported and analyzed in parallel with