tailieunhanh - Báo cáo khoa học: Lumican expression is positively correlated with the differentiation and negatively with the growth of human osteosarcoma cells

Osteosarcoma is the most common primary bone tumour associated with childhood and adolescence. The possible role of the small leucine-rich pro-teoglycan, lumican, in the growth and metastasis of various cancer types has recently been investigated. | ễFEBS Journal Lumican expression is positively correlated with the differentiation and negatively with the growth of human osteosarcoma cells Dragana Nikitovic1 Aikaterini Berdiaki1 Alexandros Zafiropoulos1 Pavlos Katonis2 Aristidis Tsatsakis1 Nikos K. Karamanos3 and George N. Tzanakakis1 1 Department of Histology MedicalSchool University of Crete Heraklion Greece 2 Department of Orthopaedics University Hospitalof Heraklion Medical School University of Crete Heraklion Greece 3 Laboratory of Biochemistry Department of Chemistry University of Patras Greece Keywords extracellular matrix osteosarcoma proteoglycans smallleucine-rich proteoglycan Correspondence G. N. Tzanakakis Department of Histology Medical School University of Crete 71110 Heraklion Greece Fax Tel 30 2810 394719 E-mail tzanakak@ Received 27 July 2007 revised 2 November 2007 accepted 23 November 2007 doi Osteosarcoma is the most common primary bone tumour associated with childhood and adolescence. The possible role of the small leucine-rich proteoglycan lumican in the growth and metastasis of various cancer types has recently been investigated. In this study the expression of lumican was examined in moderately differentiated MG-63 and well-differentiated Saos 2 human osteosarcoma cell lines of high and low metastatic capability respectively. Real-time PCR western blotting with antibodies against the protein core and keratan sulfate and specific enzymatic digestions were the methods employed. The two human osteosarcoma cell lines were found to express and secrete lumican partly substituted with keratan sulfate glycosaminoglycans. Importantly the non-metastatic well-differentiated Saos 2 cells produced lumican at rates that were up to sevenfold higher than those of highly metastatic MG-63 cells. The utilization of short interfering RNA specific for the lumican gene resulted in efficient down-regulation of its mRNA levels in both cell lines. The growth of Saos 2 .

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