tailieunhanh - Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching

The nuage is a unique organelle in animal germ cells that is known as an electron-dense amorphous structure in the perinuclear region. Although the nuage is essential for primordial germ cell (PGC) determination and devel-opment, its roles and functions are poorly understood. | ỊFEBS Journal Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching Issei Nagao1 Yumiko Aoki2 Minoru Tanaka2 and Masataka Kinjo1 1 Laboratory of Molecular CellDynamics Faculty of Advanced Life Science Hokkaido University Sapporo Japan 2 Laboratory of Molecular Genetics for Reproduction NationalInstitute for Basic Biology Okazaki Japan Keywords fluorescence correlation spectroscopy fluorescence recovery after photobleaching medaka primordialgerm cell vasa Correspondence M. Kinjo Laboratory of Molecular Cell Dynamics Faculty of Advanced Life Science Hokkaido University Kita 21 Nishi 11 Kita-ku Sapporo 001-0021 Japan Fax 81 1 706 9006 Tel 81 1 706 9005 E-mail kinjo@ Present address BiologicalInformation Research Center National institute of Advanced Industrial Science and Technology AIST and Japan Biologicalinformatics Consortium JBIC Tokyo Japan Database DNA data bank of Japan accession numbers olvas AB063484 nanos3 AB306931 tudor AB306932 Received 27 June 2007 revised 13 November 2007 accepted 21 November 2007 The nuage is a unique organelle in animal germ cells that is known as an electron-dense amorphous structure in the perinuclear region. Although the nuage is essential for primordial germ cell PGC determination and development its roles and functions are poorly understood. Herein we report an analysis of the diffusion properties of the olvas gene product of the medaka fish Oryzias lapites in PGCs prepared from embryos using fluorescence correlation spectroscopy and fluorescence recovery after photobleaching. Olvas-green fluorescent protein GFP localized in granules thought to be nuages and exhibited a constraint movement with two-component diffusion constants of and Jirrs 1. On the other hand cytosolic Olvas-GFP was also observed to have a diffusion movement of pm2-s-1. Interestingly Olvas-GFP could be expressed in HeLa cells and .