tailieunhanh - Báo cáo hóa học: " Characterization of diverse natural variants of CYP102A1 found within a species of Bacillus megaterium"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Characterization of diverse natural variants of CYP102A1 found within a species of Bacillus megaterium | Kang et al. AMB Express 2011 1 1 http content 1 1 1 o AMB Express a SpringerOpen Journal ORIGINAL ARTICLE Open Access Characterization of diverse natural variants of CYP102A1 found within a species of Bacillus megaterium 1 f 1 f 1 1 1 1 1 Ji-Yeon Kang So-Young Kim Dooil Kim Dong-Hyun Kim Sun-Mi Shin Sun-Ha Park Keon-Hee Kim 2 2 1 -i-z-i 1 1 3 1 Heung-Chae Jung Jae-Gu Pan Young Hee Joung Youn-Tae Chi Ho Zoon Chae Taeho Ahn Chul-Ho Yun Abstract An extreme diversity of substrates and catalytic reactions of cytochrome P450 P450 enzymes is considered to be the consequence of evolutionary adaptation driven by different metabolic or environmental demands. Here we report the presence of numerous natural variants of P450 BM3 CYP102A1 within a species of Bacillus megaterium. Extensive amino acid substitutions up to 5 of the total 1049 amino acid residues were identified from the variants. Phylogenetic analyses suggest that this P450 gene evolve more rapidly than the rRNA gene locus. It was found that key catalytic residues in the substrate channel and active site are retained. Although there were no apparent variations in hydroxylation activity towards myristic acid C14 and palmitic acid C16 the hydroxylation rates of lauric acid C12 by the variants varied in the range of 25-fold. Interestingly catalytic activities of the variants are promiscuous towards non-natural substrates including human P450 substrates. It can be suggested that CYP102A1 variants can acquire new catalytic activities through site-specific mutations distal to the active site. Introduction Cytochrome P450S EC P450 or CYP are remarkably diverse oxygenation catalysts that are found throughout all classes of life. Although over 11 200 genes of P450s have been found in archaea bacteria fungi plants and animals the Cytochrome P450 homepage http their evolution is not clear. An extreme diversity of substrates and catalytic reactions is .