tailieunhanh - PROTEIN ENGINEERING

Other methods have proposed the use of standard beads (Polysciences latex beads), as well described by Gasol and del Giorgio in 2000. Briefly, the beads have to be counted every day and must be sonicated to avoid aggregation. Flow rate calibration can be performed by weighing a tube containing water, processing various volumes, estimating the time needed for each volume to go through and then reweighing the tube. This makes it possible to calculate the mean of the flow rate per minute (Paul, 2001). The third method is comprised of estimating volume differences: the volume of the sample is measured by a micro-pipette before and after the run. | PROTEIN ENGINEERING Edited by Pravin Kaumaya Protein Engineering Edited by Pravin Kaumaya Published by InTech Janeza Trdine 9 51000 Rijeka Croatia Copyright 2012 InTech All chapters are Open Access distributed under the Creative Commons Attribution license which allows users to download copy and build upon published articles even for commercial purposes as long as the author and publisher are properly credited which ensures maximum dissemination and a wider impact of our publications. After this work has been published by InTech authors have the right to republish it in whole or part in any publication of which they are the author and to make other personal use of the work. Any republication referencing or personal use of the work must explicitly identify the original source. As for readers this license allows users to download copy and build upon published chapters even for commercial purposes as long as the author and publisher are properly credited which ensures maximum dissemination and a wider impact of our publications. Notice Statements and opinions expressed in the chapters are these of the individual contributors and not necessarily those of the editors or publisher. No responsibility is accepted for the accuracy of information contained in the published chapters. The publisher assumes no responsibility for any damage or injury to persons or property arising out of the use of any materials instructions methods or ideas contained in the book. Publishing Process Manager Molly Kaliman Technical Editor Teodora Smiljanic Cover Designer InTech Design Team First published February 2012 Printed in Croatia A free online edition of this book is available at Additional hard copies can be obtained from orders@ Protein Engineering Edited by Pravin Kaumaya p. cm. ISBN 978-953-51-0037-9 INTECH open science I open minds free online editions of InTech Books and Journals can be found at .