tailieunhanh - CONGENITAL ANOMALIES – CASE STUDIES AND MECHANISMS

The second chapter discusses how DNA bisulfite treatment together with high-throughput sequencing allows determining the DNA methylation on a whole genome scale at single cytosine resolution and introduces software for analysis of bisulfite sequencing data. The third chapter presents analysis of GC3-rich genes that have more methylation targets. The fourth chapter is dedicated to inheritance of DNA methylation in plant genomes and introduces restriction landmark genome scanning method - a quantitative approach for simultaneous assay of methylation status and the fifth chapter presents MethylMeter, a new bisulfite-free method to detect and quantify DNA methylation is described and applied to the detection of imprinting disorders | CONGENITAL ANOMALIES - CASE STUDIES AND MECHANISMS Edited by Alastair Sutcliffe Congenital Anomalies - Case Studies and Mechanisms Edited by Alastair Sutcliffe Published by InTech Janeza Trdine 9 51000 Rijeka Croatia Copyright 2012 InTech All chapters are Open Access distributed under the Creative Commons Attribution license which allows users to download copy and build upon published articles even for commercial purposes as long as the author and publisher are properly credited which ensures maximum dissemination and a wider impact of our publications. After this work has been published by InTech authors have the right to republish it in whole or part in any publication of which they are the author and to make other personal use of the work. Any republication referencing or personal use of the work must explicitly identify the original source. As for readers this license allows users to download copy and build upon published chapters even for commercial purposes as long as the author and publisher are properly credited which ensures maximum dissemination and a wider impact of our publications. Notice Statements and opinions expressed in the chapters are these of the individual contributors and not necessarily those of the editors or publisher. No responsibility is accepted for the accuracy of information contained in the published chapters. The publisher assumes no responsibility for any damage or injury to persons or property arising out of the use of any materials instructions methods or ideas contained in the book. Publishing Process Manager Ana Skalamera Technical Editor Teodora Smiljanic Cover Designer InTech Design Team First published February 2012 Printed in Croatia A free online edition of this book is available at Additional hard copies can be obtained from orders@ Congenital Anomalies - Case Studies and Mechanisms Edited by Alastair Sutcliffe p. cm. ISBN 978-953-51-0075-1 INTECH open science I open minds

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