tailieunhanh - Báo cáo y học: " Viral particles of the endogenous retrovirus ZAM from Drosophila melanogaster use a pre-existing "

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài:Viral particles of the endogenous retrovirus ZAM from Drosophila melanogaster use a pre-existing | Retrovirology BioMed Central Research Open Access Viral particles of the endogenous retrovirus ZAM from Drosophila melanogaster use a pre-existing endosome exosome pathway for transfer to the oocyte E Brasset1 ARTaddei2 F Arnaud1 B Faye1 AM Fausto2 M Mazzini2 F Giorgi 2 and C Vaury 1 Address 1INSERM U384 Faculté de Médecine BP38 63001 Clermont-Ferrand France and 2Centre of Electron Microscopy Department of Environmental Sciences Tuscia University Viterbo Italy Email E Brasset- ARTaddei - artaddei@ F Arnaud - B Faye - bab_faye@ AM Fausto - fausto@ M Mazzini - mazzini@ F Giorgi - giorgif@ CVaury - Corresponding authors Published 09 May 2006 Received 05 January 2006 Retrovirology 2006 3 25 doi l 742-4690-3-25 Accepted 09 May 2006 This article is available from http content 3 1 25 2006 Brasset et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Retroviruses have evolved various mechanisms to optimize their transfer to new target cells via late endosomes. Here we analyzed the transfer of ZAM a retroelement from Drosophila melanogaster from ovarian follicle cells to the oocyte at stage 9-10 of oogenesis when an active yolk transfer is occurring between these two cell types. Results Combining genetic and microscopic approaches we show that a functional secretory apparatus is required to tether ZAM to endosomal vesicles and to direct its transport to the apical side of follicle cells. There ZAM egress requires an intact follicular epithelium communicating with the oocyte. When gap junctions are inhibited or yolk receptors