tailieunhanh - Báo cáo y học: " Kinetic studies of HIV-1 and HIV-2 envelope glycoprotein-mediated fusion"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: " Kinetic studies of HIV-1 and HIV-2 envelope glycoprotein-mediated fusion. | Retrovirology BioMed Central Research Open Access Kinetic studies of HIV-1 and HIV-2 envelope glycoprotein-mediated fusion Stephen A Gallo1 Jacqueline D Reeves2 Himanshu Garg1 Brian Foley3 Robert W Doms2 and Robert Blumenthal 1 Address 1Center for Cancer Research Nanobiology Program National Cancer Institute at Frederick National Institutes of Health Frederick MD USA 2Dept. Microbiology University of Pennsylvania Philadelphia PA USA and 3Los Alamos National Laboratories Los Alamos NM USA Email Stephen A Gallo - sgallo@ Jacqueline D Reeves - jreeves@ Himanshu Garg - gargh@ Brian Foley - btf@ Robert W Doms - doms@ Robert Blumenthal - blumen@ Corresponding author Published 04 December 2006 Received 13 July 2006 Accepted 04 December 2006 Retrovirology 2006 3 90 doi 1742-4690-3-90 This article is available from http content 3 1 90 2006 Gallo et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background HIV envelope glycoprotein Env -mediated fusion is driven by the concerted coalescence of the HIV gp41 N-helical and C-helical regions which results in the formation of 6 helix bundles. Kinetics of HIV Env-mediated fusion is an important determinant of sensitivity to entry inhibitors and antibodies. However the parameters that govern the HIV Env fusion cascade have yet to be fully elucidated. We address this issue by comparing the kinetics HIV-1IIIB Env with those mediated by HIV-2 from two strains with different affinities for CD4 and CXCR4. Results HIV-1 and HIV-2 Env-mediated cell fusion occurred with half times of about 60 and 30 min respectively. Binding experiments of soluble HIV gp120 proteins to CD4 and .