tailieunhanh - Báo cáo y học: " Modification of the Tet-On regulatory system prevents the conditional-live HIV-1 variant from losing doxycycline-control"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: " Modification of the Tet-On regulatory system prevents the conditional-live HIV-1 variant from losing doxycycline-control. | Retrovirology BioMed Central Open Access Modification of the Tet-On regulatory system prevents the conditional-live HIV-1 variant from losing doxycycline-control Xue Zhou Monique Vink Ben Berkhout and Atze T Das Address Laboratory of Experimental Virology Department of Medical Microbiology Center for Infection and Immunity Amsterdam CINIMA Academic Medical Center of the University of Amsterdam Meibergdreef 15 1105 AZ Amsterdam The Netherlands Email Xue Zhou - Monique Vink - Ben Berkhout - Atze T Das - Corresponding author Published 09 November 2006 Received 25 August 2006 Accepted 09 November 2006 Retrovirology 2006 3 82 doi l 742-4690-3-82 This article is available from http content 3 1 82 2006 Zhou et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background We have previously constructed a doxycycline dox -dependent HIV-1 variant by incorporating the Tet-On gene regulatory system into the viral genome. Replication of this HIV-rtTA virus is driven by the dox-inducible transactivator protein rtTA and can be switched on and off at will. We proposed this conditional-live virus as a novel vaccine approach against HIV-1. Upon vaccination replication of HIV-rtTA can be temporarily activated by transient dox administration and controlled to the extent needed for optimal induction of the immune system. However subsequent dox-withdrawal may impose a selection for virus variants with reduced dox-dependence. Results We simulated this on off switching of virus replication in multiple independent cultures and could indeed select for HIV-rtTA variants that replicated without dox. Nearly all evolved variants had .