tailieunhanh - Báo cáo y học: " Processing sites in the human immunodeficiency virus type 1 (HIV-1) Gag-Pro-Pol precursor are cleaved by the viral protease at different rates"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Processing sites in the human immunodeficiency virus type 1 (HIV-1) Gag-Pro-Pol precursor are cleaved by the viral protease at different rates | Retrovirology BioMed Central Short report Open Access Processing sites in the human immunodeficiency virus type I HIV-1 Gag-Pro-Pol precursor are cleaved by the viral protease at different rates Steve C Pettit1 3 6 Jeffrey N Lindquist2 5 Andrew H Kaplan1 and Ronald Swanstrom 2 3 4 Address Department of Medicine University of North Carolina at Chapel Hill Chapel Hill NC USA 2Department of Biochemistry and Biophysics University of North Carolina at Chapel Hill Chapel Hill NC USA 3The UNC Center for AIDS Research University of North Carolina at Chapel Hill Chapel Hill NC USA 4CB7295 Rm 22-006 Lineberger Bldg UNC Center For AIDS Research University of North Carolina at Chapel Hill Chapel Hill NC 27599-7295 USA 5Department of Pathology Moores UCSD Cancer Center 3855 Health Sciences Dr. 0803 La Jolla CA 92093-0803 USA and 63805-103 Chimney Ridge Pl. Durham NC 27713 USA Email Steve C Pettit - stpettit@ Jeffrey N Lindquist - jlindquist@ Andrew H Kaplan - akaplan@ Ronald Swanstrom - risunc@ Corresponding author Published 01 November 2005 Received 02 August 2005 Accepted 01 November 2005 Retrovirology 2005 2 66 doi l 742-4690-2-66 H This article is available from http content 2 1 66 2005 Pettit et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We have examined the kinetics of processing of the HIV-1 Gag-Pro-Pol precursor in an in vitro assay with mature protease added in trans. The processing sites were cleaved at different rates to produce distinct intermediates. The initial cleavage occurred at the p2 NC site. Intermediate cleavages occurred at similar rates at the MA CA and RT IN sites and to a lesser extent at sites upstream of RT. Late cleavages .